Biochemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt, 5th settlement, Cairo, Egypt.
Cardiology Department, Faculty of Medicine, Al-Azhar University, Nasr city, Cairo, Egypt.
PLoS One. 2018 Dec 17;13(12):e0208038. doi: 10.1371/journal.pone.0208038. eCollection 2018.
C1q complement/tumor necrosis factor (TNF)-related protein (CTRP) family comprises of 15 proteins that posses important implications in energy homeostasis, infection and inflammation. However, their roles in diabetes mellitus (DM) and its vascular complications have not been completely assessed. This works aims to study the association of two CTRPs; 3 and 9, with pro-inflammatory cytokine monocyte chemoattractant protein-1 (MCP-1), and biochemical parameters of type 2 diabetes (T2D), dyslipidemia and coronary artery disease (CAD).
Biochemical markers and serum levels of CTRPs and MCP-1 were measured in 86 postmenopausal females. Subjects were divided over four groups; 13 apparent healthy subjects as control (group I), 29 patients with CAD (group II), 29 patients with T2D ≥5 years (group III) and 15 patients with CAD secondary to T2D (group IV). Serum CTRP3, CTRP9, MCP-1 and insulin were measured by ELISA.
Serum CTRP3 levels were found to be significantly higher in group III and IV, whereas, it was significantly lower in group II on comparing to group I. While, CTRP9 levels were significantly decreased in group II, III and IV on comparing to group I. MCP-1 levels were found to be significantly increased in groups II, III and IV on comparison with group I. Both CTRPs were significantly negatively correlated with each other. While MCP-1 was significantly correlated negatively to CTRP9.
This study associates the possible role of CTRP3, CTRP9 and MCP-1/CCL2 in the diagnosis/prognosis of CAD complication in T2D postmenopausal females.
C1q 补体/肿瘤坏死因子（TNF）相关蛋白（CTRP）家族由 15 种蛋白质组成，这些蛋白质在能量平衡、感染和炎症中具有重要意义。然而，它们在糖尿病（DM）及其血管并发症中的作用尚未完全评估。这项工作旨在研究两种 CTRP；3 和 9，与促炎细胞因子单核细胞趋化蛋白-1（MCP-1）以及 2 型糖尿病（T2D）、血脂异常和冠心病（CAD）的生化参数的关系。
在 86 名绝经后女性中测量了 CTRPs 和 MCP-1 的生化标志物和血清水平。受试者分为四组；13 名明显健康的受试者作为对照组（I 组）、29 名 CAD 患者（II 组）、29 名 T2D 患者（≥5 年）（III 组）和 15 名 T2D 继发 CAD 患者（IV 组）。用 ELISA 法测量血清 CTRP3、CTRP9、MCP-1 和胰岛素。
发现 III 组和 IV 组血清 CTRP3 水平明显升高，而 II 组与 I 组相比则明显降低。而 CTRP9 水平在 II、III 和 IV 组均明显低于 I 组。与 I 组相比，MCP-1 水平在 II、III 和 IV 组均明显升高。两种 CTRP 均呈显著负相关。而 MCP-1 与 CTRP9 呈显著负相关。
本研究表明，CTRP3、CTRP9 和 MCP-1/CCL2 可能在 T2D 绝经后女性 CAD 并发症的诊断/预后中起作用。
