Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China.
Int J Mol Sci. 2018 Dec 14;19(12):4062. doi: 10.3390/ijms19124062.
CDPKs (Ca-Dependent Protein Kinases) are very important regulators in plant response to abiotic stress. The molecular regulatory mechanism of CDPKs involved in salt stress tolerance remains unclear, although some CDPKs have been identified in salt-stress signaling. Here, we investigated the function of an CDPK, CPK12, in salt-stress signaling. The -RNA interference (RNAi) mutant was much more sensitive to salt stress than the wild-type plant GL1 in terms of seedling growth. Under NaCl treatment, Na⁺ levels in the roots of -RNAi plants increased and were higher than levels in GL1 plants. In addition, the level of salt-elicited H₂O₂ production was higher in -RNAi mutants than in wild-type GL1 plants after NaCl treatment. Collectively, our results suggest that CPK12 is required for plant adaptation to salt stress.
钙依赖蛋白激酶（CDPKs）在植物应对非生物胁迫中是非常重要的调节因子。尽管已经鉴定出一些 CDPKs 参与了盐胁迫信号转导，但涉及盐胁迫耐受性的 CDPKs 的分子调节机制仍不清楚。在这里，我们研究了钙依赖蛋白激酶 CPK12 在盐胁迫信号转导中的功能。在幼苗生长方面，-RNA 干扰（RNAi）突变体比野生型 GL1 对盐胁迫更为敏感。在 NaCl 处理下，-RNAi 植株的根中 Na⁺水平增加，并且高于 GL1 植株中的水平。此外，在 NaCl 处理后，-RNAi 突变体中盐诱导的 H₂O₂产生水平高于野生型 GL1 植株。总的来说，我们的结果表明，CPK12 是植物适应盐胁迫所必需的。
