State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2018 Dec 15;19(12):4065. doi: 10.3390/ijms19124065.
Plant immune activators can protect crops from plant virus pathogens by activating intrinsic immune mechanisms in plants and are widely used in agricultural production. In our previous work, we found that curcumin analogs exhibit excellent biological activity against plant viruses, especially protective activity. Inspired by these results, the active substructure of pentadienone and quinazoline were spliced to obtain curcumin analogs as potential exogenously induced resistant molecule. Bioassay results showed that compound exhibited excellent protective activity for tobacco to against Tobacco mosaic virus (TMV) at 500 μg/mL, with a value of 70.4 ± 2.6% compared with control treatments, which was better than that of the plant immune activator chitosan oligosaccharide (49.0 ± 5.9%). The protective activity is due to compound inducing tobacco resistance to TMV, which was related to defense-related enzymes, defense-related genes, and photosynthesis. This was confirmed by the up-regulated expression of proteins that mediate stress responses and oxidative phosphorylation.
植物免疫激活剂可以通过激活植物内在的免疫机制来保护作物免受植物病毒病原体的侵害，因此被广泛应用于农业生产中。在我们之前的工作中，我们发现姜黄素类似物对植物病毒具有优异的生物活性，尤其是保护活性。受这些结果的启发，我们将戊二烯酮和喹唑啉的活性亚结构拼接在一起，得到了作为潜在的外源诱导抗性分子的姜黄素类似物。生物测定结果表明，化合物在 500μg/mL 时对烟草表现出优异的保护活性，对烟草花叶病毒（TMV）的保护活性值为 70.4±2.6%，与对照处理相比，优于植物免疫激活剂壳寡糖（49.0±5.9%）。这种保护活性是由于化合物诱导烟草对 TMV 的抗性，这与防御相关酶、防御相关基因和光合作用有关。这一点通过介导应激反应和氧化磷酸化的蛋白质的上调表达得到了证实。
