Bikel Shirley, López-Leal Gamaliel, Cornejo-Granados Fernanda, Gallardo-Becerra Luigui, García-López Rodrigo, Sánchez Filiberto, Equihua-Medina Edgar, Ochoa-Romo Juan Pablo, López-Contreras Blanca Estela, Canizales-Quinteros Samuel, Hernández-Reyna Abigail, Mendoza-Vargas Alfredo, Ochoa-Leyva Adrian
Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Avenida Universidad 2001, Cuernavaca, Morelos 62210, Mexico.
Unidad de Genomica de Poblaciones Aplicada a la Salud, Facultad de Quimica, UNAM/Instituto Nacional de Medicina Genomica (INMEGEN), Mexico City, Mexico.
iScience. 2021 Jul 24;24(8):102900. doi: 10.1016/j.isci.2021.102900. eCollection 2021 Aug 20.
Changes in the human gut microbiome are associated with obesity and metabolic syndrome, but the role of the gut virome in both diseases remains largely unknown. We characterized the gut dsDNA virome of 28 school-aged children with healthy normal-weight (NW, n = 10), obesity (O, n = 10), and obesity with metabolic syndrome (OMS, n = 8), using metagenomic sequencing of virus-like particles (VLPs) from fecal samples. The virome classification confirmed the bacteriophages' dominance, mainly composed of Caudovirales. Notably, phage richness and diversity of individuals with O and OMS tended to increase, while the VLP abundance remained the same among all groups. Of the 4,611 phage contigs composing the phageome, 48 contigs were highly prevalent in ≥80% of individuals, suggesting high inter-individual phage diversity. The abundance of several contigs correlated with gut bacterial taxa; and with anthropometric and biochemical parameters altered in O and OMS. To our knowledge, this gut phageome represents one of the largest datasets and suggests disease-specific phage alterations.
人类肠道微生物群的变化与肥胖和代谢综合征有关，但肠道病毒组在这两种疾病中的作用仍 largely 未知。我们使用粪便样本中病毒样颗粒（VLP）的宏基因组测序，对 28 名学龄儿童的肠道双链 DNA 病毒组进行了特征分析，这些儿童分别为健康正常体重（NW，n = 10）、肥胖（O，n = 10）和患有代谢综合征的肥胖儿童（OMS，n = 8）。病毒组分类证实了噬菌体的主导地位，主要由有尾噬菌体目组成。值得注意的是，肥胖和患有代谢综合征的个体的噬菌体丰富度和多样性趋于增加，而所有组中的 VLP 丰度保持不变。在构成噬菌体组的 4611 个噬菌体重叠群中，48 个重叠群在≥80%的个体中高度普遍，表明个体间噬菌体多样性很高。几个重叠群的丰度与肠道细菌分类群相关；并且与肥胖和患有代谢综合征的个体中改变的人体测量和生化参数相关。据我们所知，这个肠道噬菌体组代表了最大的数据集之一，并表明了特定疾病的噬菌体改变。
