低聚半乳糖通过调节脂多糖刺激仔猪的黏膜相关微生物群组成来改善屏障功能并减轻结肠炎症。

Galacto-oligosaccharides improve barrier function and relieve colonic inflammation via modulating mucosa-associated microbiota composition in lipopolysaccharides-challenged piglets.

作者信息

Gao Ren, Tian Shiyi, Wang Jing, Zhu Weiyun

机构信息

National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, National Experimental Teaching Demonstration Center of Animal Science, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

出版信息

J Anim Sci Biotechnol. 2021 Aug 11;12(1):92. doi: 10.1186/s40104-021-00612-z.

DOI:10.1186/s40104-021-00612-z

PMID:34376253

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8356462/

Abstract

BACKGROUND

Galacto-oligosaccharides (GOS) have been shown to modulate the intestinal microbiota of suckling piglets to exert beneficial effects on intestinal function. However, the modulation of intestinal microbiota and intestinal function by GOS in intestinal inflammation injury models has rarely been reported. In this study, we investigated the effects of GOS on the colonic mucosal microbiota composition, barrier function and inflammatory response of lipopolysaccharides (LPS)-challenged suckling piglets.

METHODS

A total of 18 newborn suckling piglets were divided into three groups, the CON group, the LPS-CON group and the LPS-GOS group. Piglets in the LPS-GOS group were orally fed with 1 g/kg body weight of GOS solution every day. On the d 14, piglets in the LPS-CON and LPS-GOS group were challenged intraperitoneally with LPS solution. All piglets were slaughtered 2 h after intraperitoneal injection and sampled.

RESULTS

We found that the colonic mucosa of LPS-challenged piglets was significantly injured and shedding, while the colonic mucosa of the LPS-GOS group piglets maintained its structure. Moreover, GOS significantly reduced the concentration of malondialdehyde (MDA) and the activity of reactive oxygen species (ROS) in the LPS-challenged suckling piglets, and significantly increased the activity of total antioxidant capacity (T-AOC). GOS significantly increased the relative abundance of norank_f__Muribaculaceae and Romboutsia, and significantly decreased the relative abundance of Alloprevotella, Campylobacter and Helicobacter in the colonic mucosa of LPS-challenged suckling piglets. In addition, GOS increased the concentrations of acetate, butyrate and total short chain fatty acids (SCFAs) in the colonic digesta of LPS-challenged suckling piglets. GOS significantly reduced the concentrations of interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and cluster of differentiation 14 (CD14), and the relative mRNA expression of Toll-like receptor 4 (TLR4) and myeloid differentiation primary response 88 (MyD88) in the LPS-challenged suckling piglets. In addition, GOS significantly reduced the relative mRNA expression of mucin2 (MUC2), and significantly increased the protein expression of Claudin-1 and zonula occluden-1 (ZO-1) in LPS-challenged suckling piglets.

CONCLUSIONS

These results suggested that GOS can modulate the colonic mucosa-associated microbiota composition and improve the intestinal function of LPS-challenged suckling piglets.

摘要

背景

低聚半乳糖（GOS）已被证明可调节哺乳仔猪的肠道微生物群，对肠道功能产生有益影响。然而，在肠道炎症损伤模型中，GOS对肠道微生物群和肠道功能的调节作用鲜有报道。在本研究中，我们调查了GOS对脂多糖（LPS）攻击的哺乳仔猪结肠黏膜微生物群组成、屏障功能和炎症反应的影响。

方法

将18只新生哺乳仔猪分为三组，即对照组（CON组）、LPS对照组（LPS-CON组）和LPS-GOS组。LPS-GOS组仔猪每天口服1 g/kg体重的GOS溶液。在第14天，LPS-CON组和LPS-GOS组仔猪腹腔注射LPS溶液进行攻击。所有仔猪在腹腔注射后2小时屠宰并取样。

结果

我们发现，LPS攻击的仔猪结肠黏膜明显受损且脱落，而LPS-GOS组仔猪的结肠黏膜结构保持完整。此外，GOS显著降低了LPS攻击的哺乳仔猪中丙二醛（MDA）的浓度和活性氧（ROS）的活性，并显著提高了总抗氧化能力（T-AOC）的活性。GOS显著增加了LPS攻击的哺乳仔猪结肠黏膜中无分类单元_f__毛螺菌科和罗姆布茨菌属的相对丰度，并显著降低了别普雷沃菌属、弯曲杆菌属和幽门螺杆菌属的相对丰度。此外，GOS增加了LPS攻击的哺乳仔猪结肠内容物中乙酸、丁酸和总短链脂肪酸（SCFA）的浓度。GOS显著降低了LPS攻击的哺乳仔猪中白细胞介素1β（IL-1β）、白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）和分化抗原簇14（CD14）的浓度，以及Toll样受体4（TLR4）和髓样分化初级反应88（MyD88）的相对mRNA表达。此外，GOS显著降低了LPS攻击的哺乳仔猪中黏蛋白2（MUC2）的相对mRNA表达，并显著增加了Claudin-1和紧密连接蛋白1（ZO-1）的蛋白表达。