Department of Biology, University of Pisa, via San Zeno 31, 56127 Pisa, Italy.
Interdepartmental Research Center Nutrafood "Nutraceuticals and Food for Health", University of Pisa, via del Borghetto 80, 56124 Pisa, Italy.
Nutrients. 2018 Dec 5;10(12):1932. doi: 10.3390/nu10121932.
Lisosan G (LG), a fermented powder obtained from whole grains, is a recognized antioxidant compound that improves the bioactivity and survival of different cell types. The purpose of this study was to investigate whether LG ameliorates both the neural and the vascular damage characterizing early stages of diabetic retinopathy (DR). The effects of LG were studied in cultured explants of mouse retinas challenged with oxidative stress (OS) or in retinas of streptozotocin (STZ)-treated rats. Apoptosis, vascular endothelial growth factor (VEGF) expression, OS markers, blood-retinal barrier (BRB) integrity, and inflammation were assessed, while retinal function was evaluated with electroretinogram (ERG). LG extensively inhibited apoptosis, VEGF expression, and OS both in retinal explants and in STZ rats. In addition, STZ rats treated with LG displayed an almost total BRB integrity, reduced levels of inflammatory markers and a partially restored visual function as evaluated with ERG. In summary, we demonstrated that LG exhibits antioxidant and anti-inflammatory effects that exert powerful protective actions against neural and vascular defects characteristic of DR. Therefore, LG-containing foods or supplements may be considered to implement DR treatments.
Lisosan G（LG）是一种从全谷物中提取的发酵粉末，是一种公认的抗氧化化合物，可提高不同细胞类型的生物活性和存活率。本研究旨在探讨 LG 是否可以改善糖尿病视网膜病变（DR）早期的神经和血管损伤。LG 的作用在受到氧化应激（OS）挑战的培养的小鼠视网膜外植体或链脲佐菌素（STZ）处理的大鼠视网膜中进行了研究。评估了细胞凋亡、血管内皮生长因子（VEGF）表达、OS 标志物、血视网膜屏障（BRB）完整性和炎症，同时用电视网膜图（ERG）评估视网膜功能。LG 广泛抑制了视网膜外植体和 STZ 大鼠中的细胞凋亡、VEGF 表达和 OS。此外，用 LG 治疗的 STZ 大鼠显示出几乎完整的 BRB 完整性、炎症标志物水平降低以及部分恢复的视觉功能，可通过 ERG 评估。总之，我们证明 LG 具有抗氧化和抗炎作用，可对 DR 特征性的神经和血管缺陷发挥强大的保护作用。因此，含有 LG 的食物或补充剂可用于实施 DR 治疗。
