Chatzifrangkeskou Maria, O'Neill Eric
Department of Oncology, University of Oxford, Oxford, UK.
Methods Mol Biol. 2019;1893:115-119. doi: 10.1007/978-1-4939-8910-2_10.
The translocation or shuttling of Hippo proteins between the nucleus and cytoplasm is a rapid event following cytoskeletal or mechanical cues as well as stimulation with extracellular growth factors. Here we describe an experimental procedure for a simple and fast separation of nuclear and cytoplasmic fractions which maintains protein integrity and integrity of protein-protein complexes, indicating that it should be applicable to many experimental questions.
在细胞骨架或机械信号以及细胞外生长因子刺激后，Hippo蛋白在细胞核和细胞质之间的转运或穿梭是一个快速事件。在此，我们描述了一种简单快速分离细胞核和细胞质组分的实验方法，该方法能保持蛋白质完整性以及蛋白质-蛋白质复合物的完整性，这表明它应适用于许多实验问题。
