研究 PCL-胶原纳米纤维中胶原的批间不一致性。
Investigation of the batch-to-batch inconsistencies of Collagen in PCL-Collagen nanofibers.
机构信息
Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen 91058, Germany; Department of Plastic- and Hand Surgery, University Hospital of Erlangen, 91052 Erlangen, Germany.
Department of Plastic- and Hand Surgery, University Hospital of Erlangen, 91052 Erlangen, Germany.
出版信息
Mater Sci Eng C Mater Biol Appl. 2019 Feb 1;95:217-225. doi: 10.1016/j.msec.2018.10.057. Epub 2018 Oct 26.
The application of tissue engineered constructs is an approach in regenerative therapies to support regeneration of damaged muscular tissue. Therefore the development of highly aligned electrospun scaffolds based on polycaprolactone (PCL) and collagen enables a versatile development towards tailor made applications. However, the application of natural polymers like collagen brings the risk of batch-to-batch inconsistencies, which influence the reproducibility of the electrospinning process. Aligned PCL-Collagen nanofibers were fabricated via electrospinning using benign solvents. The spinnability of different collagen batches and polymer concentrations in diluted acetic acid as solvent was investigated. Furthermore spinning parameters and fiber morphology were investigated in order to determine the most stable spinning conditions and analyze the batch-to-batch variations. Finally the effect of the solution temperature and the time of pure collagen in solution were investigated, to complete the analysis of the influences on the spinning behavior.
组织工程构建的应用是再生治疗中的一种方法，可用于支持受损肌肉组织的再生。因此，基于聚己内酯（PCL）和胶原蛋白的高度取向电纺支架的开发能够实现针对定制应用的多功能开发。然而，使用胶原等天然聚合物带来了批次不一致的风险，这会影响到电纺过程的重现性。通过使用良性溶剂的静电纺丝技术制造了 PCL-胶原纳米纤维。研究了不同胶原批次和聚合物浓度在稀释乙酸溶剂中的可纺性。此外，还研究了纺丝参数和纤维形态，以确定最稳定的纺丝条件，并分析批间变化。最后，研究了溶液温度和纯胶原蛋白在溶液中的时间的影响，以完成对纺丝行为影响的分析。
Zotero 插件