Dippold Dirk, Cai Aijia, Hardt Moritz, Boccaccini Aldo R, Horch Raymund, Beier Justus P, Schubert Dirk W
Institute of Polymer Materials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany; Department of Plastic- and Hand Surgery, University Hospital of Erlangen, 91058 Erlangen, Germany.
Department of Plastic- and Hand Surgery, University Hospital of Erlangen, 91058 Erlangen, Germany.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:278-283. doi: 10.1016/j.msec.2016.11.045. Epub 2016 Nov 24.
Under several conditions such as severe trauma skeletal muscle lack the ability to reorganize and the loss of muscle function is inevitable. The application of tissue engineered constructs is a promising approach in regenerative therapies for damaged muscular tissue. Therefore, the development of highly aligned scaffolds based on polycaprolactone (PCL) has been studied extensively. Nanofiber scaffolds containing collagen have mostly been fabricated via electrospinning using highly corrosive 1,1,1,3,3,3 hexafluoro-2-propanol (HFIP) so far. In this study, aligned Polycaprolactone-Collagen (PCL-Coll) biocomposite nanofibers were fabricated via electrospinning using environmentally benign diluted acetic acid (AcOH) as solvent. Furthermore, ultrasonic treatment was introduced to enhance the intrinsically weak solubility of PCL in AcOH. AcOH was diluted to an ideal concentration for electrospinning of 90%. The final solutions were spun at various conditions and collected with different collector setups in order to determine ideal processing conditions for the fabrication of highly aligned nanofibers.
在诸如严重创伤等多种情况下，骨骼肌缺乏重组能力，肌肉功能丧失不可避免。组织工程构建体的应用是受损肌肉组织再生治疗中一种很有前景的方法。因此，基于聚己内酯（PCL）的高度排列支架的开发已得到广泛研究。迄今为止，含胶原蛋白的纳米纤维支架大多是通过使用高腐蚀性的1,1,1,3,3,3-六氟-2-丙醇（HFIP）进行静电纺丝制备的。在本研究中，使用环境友好的稀释乙酸（AcOH）作为溶剂，通过静电纺丝制备了排列的聚己内酯-胶原蛋白（PCL-Coll）生物复合纳米纤维。此外，引入超声处理以增强PCL在AcOH中固有的低溶解度。将AcOH稀释至90%这一静电纺丝的理想浓度。在不同条件下纺丝最终溶液，并采用不同的收集器设置进行收集，以确定制备高度排列纳米纤维的理想加工条件。
