Freese J A, Sharp B L, Ngxongo S M, Markus M B
Research Institute for Diseases in a Tropical Environment of the South African Medical Research Council, Durban.
S Afr Med J. 1988 Dec 3;74(11):576-8.
In May 1987 and January 1988 the chloroquine sensitivity of Plasmodium falciparum in the Ubombo and Ingwavuma districts of KwaZulu was determined by a modified in vitro microtest in which the patients' plasma was replaced with non-immune human AB serum and the test plates were incubated in an atmosphere of 3% oxygen, 4% carbon dioxide and 93% nitrogen. A success rate of 74% was achieved using this technique. All of 23 successfully tested isolates from malaria patients reporting to clinics and a hospital in these areas were found to be resistant to chloroquine, schizogony being inhibited at 32 pmol per well in the majority of tests. Seventy-five per cent of the isolates obtained through active surveillance in the Ubombo district were found to be resistant in varying degrees. Malarial parasites collected from clinics and a hospital in the endemic area did not change markedly in their in vitro response to chloroquine during the 8-month period May 1987-January 1988.
1987年5月和1988年1月，通过一种改良的体外微量试验，测定了夸祖鲁省乌邦博和因夸武马地区恶性疟原虫对氯喹的敏感性。在该试验中，用非免疫人AB血清替代患者血浆，并将试验板置于含3%氧气、4%二氧化碳和93%氮气的环境中孵育。使用该技术的成功率为74%。在这些地区的诊所和一家医院就诊的疟疾患者中，所有23株成功检测的分离株均对氯喹耐药，在大多数试验中，每孔32皮摩尔时裂体增殖受到抑制。通过在乌邦博地区进行主动监测获得的分离株中，75%被发现有不同程度的耐药性。1987年5月至1988年1月的8个月期间，从该流行地区的诊所和一家医院收集的疟原虫对氯喹的体外反应没有明显变化。
