Brzozowa-Zasada Marlena, Kurek Józef, Piecuch Adam, Stęplewska Katarzyna
Department of Histology and Cell Pathology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Poland.
Department of Surgery, Municipal Hospital, Jaworzno, Poland.
Prz Gastroenterol. 2018;13(4):322-331. doi: 10.5114/pg.2018.79813. Epub 2018 Dec 11.
Colorectal cancer (CRC) is the third and second most commonly diagnosed cancer worldwide in males and females, respectively. Despite prominent progress in diagnosis and treatment, the recurrence rates are still high. A tumour hypoxic environment leads to an increase in glycolytic metabolism. The crucial intermediate component of glycolysis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), could play a significant role in cancer progression. An increased level of GAPDH has been described in oncogene-induced transformation and anti-apoptotic function. In other studies, GAPDH has been involved in apoptosis induction.
We examined colorectal adenocarcinoma samples to assess the immunoexpression of GAPDH protein. We also evaluated the correlation between the expression of GAPDH protein and apoptotic parameters including expression of Bcl2 and Bax.
Paraffin sections were incubated for 60 min with primary antibody against GAPDH, Bcl-2, and Bax.
Results of our study have shown that GAPDH expression in colorectal cancer is upregulated. We revealed significant positive correlation between expression of this protein and grade and size of tumour, and regional lymph node involvement. In the case of apoptosis-associated proteins, e.g. Bcl-2 and Bax, we found negative correlations between expression of these proteins and grade and size of tumour, lymphovascular invasion, and regional lymph node involvement. Finally, we demonstrated that GAPDH up-regulation is connected with down-regulation in Bcl-2 and Bax.
Up-regulation of GAPDH protein and down-regulation of Bcl-2 and Bax may result in increased of cancer.
结直肠癌(CRC)分别是全球男性和女性中第三和第二常见的诊断癌症。尽管在诊断和治疗方面取得了显著进展,但复发率仍然很高。肿瘤缺氧环境会导致糖酵解代谢增加。糖酵解的关键中间成分甘油醛-3-磷酸脱氢酶(GAPDH)可能在癌症进展中发挥重要作用。在致癌基因诱导的转化和抗凋亡功能中,GAPDH水平升高已有描述。在其他研究中,GAPDH参与了凋亡诱导。
我们检测了结直肠腺癌样本,以评估GAPDH蛋白的免疫表达。我们还评估了GAPDH蛋白表达与凋亡参数(包括Bcl2和Bax表达)之间的相关性。
将石蜡切片与抗GAPDH、Bcl-2和Bax的一抗孵育60分钟。
我们的研究结果表明,结直肠癌中GAPDH表达上调。我们发现该蛋白的表达与肿瘤分级、大小以及区域淋巴结受累之间存在显著正相关。对于凋亡相关蛋白,如Bcl-2和Bax,我们发现这些蛋白的表达与肿瘤分级、大小、淋巴管侵犯和区域淋巴结受累之间存在负相关。最后,我们证明GAPDH上调与Bcl-2和Bax下调有关。
GAPDH蛋白上调以及Bcl-2和Bax下调可能导致癌症进展。