Shoubochkina E A, Fodor I I
Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino-on-the Oka, Moscow.
Curr Genet. 1988 Sep;14(3):183-9. doi: 10.1007/BF00376738.
A genome clonotheque consisting of 25- to 40-kb Sau3AI fragments of Aspergillus terreus DNA was constructed in the episomal cosmid vector pES33 containing the yeast ARG4 gene. From the 475 transformants of cir0 yeast strain ESH-0, 23 stable Arg+ transformants were independently selected. Genetic and Southern analysis of these stable transformants showed that 39% arose as a result of recombination between cloned A. terreus DNA sequences and yeast chromosome XII. The recombination events most likely occurred in the regions of homology within the rDNA clusters of A. terreus and Saccharomyces cerevisiae.
构建了一个基因组文库，该文库由包含酵母ARG4基因的附加型黏粒载体pES33中的土曲霉DNA的25至40kb Sau3AI片段组成。从cir0酵母菌株ESH-0的475个转化子中，独立选择了23个稳定的Arg +转化子。对这些稳定转化子的遗传和Southern分析表明，39％是由于克隆的土曲霉DNA序列与酵母染色体XII之间的重组而产生的。重组事件最有可能发生在土曲霉和酿酒酵母的rDNA簇内的同源区域。
