一种用于酵母DNA克隆片段的快速染色体定位方法。
A rapid chromosome-mapping method for cloned fragments of yeast DNA.
作者信息
Falco S C, Botstein D
出版信息
Genetics. 1983 Dec;105(4):857-72. doi: 10.1093/genetics/105.4.857.
Abstract
A rapid and generally applicable method is described for mapping a cloned yeast DNA segment to the chromosome(s) from which it originated. The method is based upon the recent finding that the integration into a yeast chromosome of a segment of the 2 mu plasmid DNA results, in heterozygous diploids, in the specific loss of genetic information from the chromosome into which the 2 mu DNA was integrated (Falco et al. 1982). After verification of the accuracy of the method using several genes whose position was known in advance, the method was used to locate the yeast actin gene, which lies on the left arm of chromosome VI, about 50 cM distal to CDC4.
摘要
本文描述了一种快速且普遍适用的方法，用于将克隆的酵母DNA片段定位到其起源的染色体上。该方法基于最近的一项发现，即在杂合二倍体中，2μm质粒DNA片段整合到酵母染色体中会导致整合了2μm DNA的染色体上的遗传信息特异性丢失（Falco等人，1982年）。在使用几个预先已知位置的基因验证了该方法的准确性之后，该方法被用于定位酵母肌动蛋白基因，该基因位于第六条染色体的左臂上，距离CDC4约50厘摩。
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Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes.酵母的遗传图谱 IV. 温度敏感基因的图谱绘制以及在定位基因中的二倍体菌株的使用。
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