São Paulo State University (UNESP), Institute of Biosciences, Department of Chemistry and Biochemistry, Botucatu, SP, Brazil.
São Paulo State University (UNESP), Institute of Biosciences, Department of Chemistry and Biochemistry, Botucatu, SP, Brazil.
Food Chem. 2019 Apr 25;278:823-831. doi: 10.1016/j.foodchem.2018.11.119. Epub 2018 Nov 30.
Bioactive peptides can provide health benefits due to different mechanisms. The aims of the present study are to produce bioactive peptides from bovine and goat milk subjected to the proteolytic activity of Aspergillus oryzae and Aspergillus flavipes enzymes, as well as to assess their putative antimicrobial and antioxidant activity. Bioactive peptides were successfully generated from proteases of fungi cultivated in solid-state fermentation. The generated peptides were effective against all tested bacteria and fungi. There was antioxidant activity, up to 92.5% DPPH reduction and ORAC stabilization at 52.5 μmol μL of Trolox Equivalent. The generation of milk-specific sequences peptides in the samples was obtained through 2D-PAGE fractioning followed by mass spectrometry (ESI-MS/MS). Based on results in the present study, milk bioactive peptides presenting broad antimicrobial action and antioxidant activity spectra can be cost-effectively produced through solid-state fermentation. The herein addressed approach can be valuable for the pharmaceutical and food industries.
生物活性肽由于不同的机制可以提供健康益处。本研究的目的是从经过米曲霉和黄曲霉酶的蛋白水解活性处理的牛乳和山羊乳中生产生物活性肽，并评估它们潜在的抗菌和抗氧化活性。生物活性肽是通过固态发酵培养的真菌蛋白酶成功生成的。生成的肽对所有测试的细菌和真菌都有效。具有抗氧化活性，DPPH 还原率高达 92.5%，Trolox 当量 52.5 μmol μL 的 ORAC 稳定率。通过 2D-PAGE 分级分离和质谱（ESI-MS/MS）获得样品中乳特异性序列肽的生成。基于本研究的结果，可以通过固态发酵以具有成本效益的方式生产具有广谱抗菌作用和抗氧化活性的乳生物活性肽。本文所述的方法对于制药和食品工业可能具有重要价值。
