Krishnankutty Roopesh, Iskandarani Ahmad, Therachiyil Lubna, Uddin Shahab, Azizi Fouad, Kulinski Michael, Bhat Ajaz Ahmad, Mohammad Ramzi M
Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, State of Qatar. Email:
Asian Pac J Cancer Prev. 2018 Dec 25;19(12):3501-3509. doi: 10.31557/APJCP.2018.19.12.3501.
Background/ Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedy for various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commercially available camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines: colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects of camel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by in vitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirm autophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells. The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteins implied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosome formation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on human colorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.
背景/目的：骆驼奶传统上以对人类健康有益而闻名，并且被认为是包括癌症在内的各种人类疾病的一种治疗方法。本研究旨在评估市售骆驼奶对癌细胞的抑制作用及其潜在机制。材料与方法：使用两种细胞系：结肠直肠癌HCT 116细胞系和乳腺癌MCF - 7细胞系，用不同剂量的骆驼奶进行培养。通过MTT法测定骆驼奶对细胞死亡的影响，通过台盼蓝排斥试验测定细胞活力，通过体外划痕试验测定细胞迁移。通过蛋白质免疫印迹法阐明其机制，并用共聚焦显微镜确认自噬。结果：骆驼奶显著降低了两种细胞的增殖、活力以及迁移能力。LC3 - II蛋白的积累以及自噬蛋白p62和Atg 5 - 12表达的降低意味着自噬的诱导。共聚焦显微镜检测到的（绿色荧光蛋白）- LC3斑点证实了骆驼奶处理后自噬体的形成。结论：骆驼奶通过诱导自噬对人结肠直肠癌HCT 116细胞和乳腺癌MCF - 7细胞发挥抗增殖作用。
