Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, 10032, USA.
Integrated Program in Cellular, Molecular, and Biomedical Studies, Columbia University Irving Medical Center, New York, NY, 10032, USA.
Genome Biol. 2018 Dec 24;19(1):227. doi: 10.1186/s13059-018-1607-x.
Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We combine microfluidics with optically decodable beads and show that phenotypic analysis of living cells can be linked to single-cell sequencing. As a proof-of-concept, we demonstrate the accuracy and scalability of our tool called Single Cell Optical Phenotyping and Expression sequencing (SCOPE-Seq) to combine live cell imaging with single-cell RNA sequencing.
光学可解码珠通过光学条码将样本的身份与测量结果联系起来，从而使溶液中的生物分子文库能够在珠上捕获，并通过荧光进行解码。这种方法是微阵列、测序和基于流的表达分析技术的基础。我们将微流控技术与光学可解码珠相结合，表明可以将活细胞的表型分析与单细胞测序联系起来。作为概念验证，我们展示了我们称之为单细胞光学表型和表达测序（SCOPE-Seq）的工具的准确性和可扩展性，该工具将活细胞成像与单细胞 RNA 测序相结合。
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