College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Genes (Basel). 2022 Nov 25;13(12):2211. doi: 10.3390/genes13122211.
Single-cell sequencing (SCS) uses a single cell as the research material and involves three dimensions: genes, phenotypes and cell biological mechanisms. This type of research can locate target cells, analyze the dynamic changes in the target cells and the relationships between the cells, and pinpoint the molecular mechanism of cell formation. Currently, a common problem faced by animal husbandry scientists is how to apply existing science and technology to promote the production of high-quality livestock and poultry products and to breed livestock for disease resistance; this is also a bottleneck for the sustainable development of animal husbandry. In recent years, although SCS technology has been successfully applied in the fields of medicine and bioscience, its application in poultry science has been rarely reported. With the sustainable development of science and technology and the poultry industry, SCS technology has great potential in the application of poultry science (or animal husbandry). Therefore, it is necessary to review the innovation of SCS technology and its application in poultry science. This article summarizes the current main technical methods of SCS and its application in poultry, which can provide potential references for its future applications in precision breeding, disease prevention and control, immunity, and cell identification.
单细胞测序（SCS）以单个细胞为研究材料，涉及三个维度：基因、表型和细胞生物学机制。这种研究可以定位目标细胞，分析目标细胞的动态变化以及细胞之间的关系，并确定细胞形成的分子机制。目前，畜牧业科学家面临的一个共同问题是如何应用现有科学技术来促进高质量的畜禽产品生产和抗病畜禽的培育；这也是畜牧业可持续发展的一个瓶颈。近年来，虽然 SCS 技术已成功应用于医学和生物科学领域，但在禽类科学中的应用却鲜有报道。随着科学技术和家禽业的可持续发展，SCS 技术在禽类科学（或畜牧业）中的应用具有巨大的潜力。因此，有必要对 SCS 技术的创新及其在禽类科学中的应用进行综述。本文总结了 SCS 的当前主要技术方法及其在禽类中的应用，可为其在精准养殖、疾病防控、免疫和细胞鉴定等方面的未来应用提供潜在参考。
