College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
Int J Biol Macromol. 2019 Apr 1;126:436-442. doi: 10.1016/j.ijbiomac.2018.12.168. Epub 2018 Dec 23.
Poly(butylene succinate) (PBS) and poly(lactic acid) (PLA) were melt-blended in different proportions and selectively degraded by cutinase and proteinase K, respectively. The selective enzymatic degradation process was systematically investigated. The degraded PBS/PLA blends were analyzed via scanning electron microscopy, Fourier-transform infrared spectroscopy, powder X-ray diffraction, and differential scanning calorimetry. The results of the weight loss of PBS/PLA blends degraded by cutinase and proteinase K suggested that PLA hindered the cutinase-catalyzed degradation of PBS, whereas the addition of PBS in the blends accelerated the degradation of PLA within a specific PBS/PLA ratio. The change in crystallinity after degradation was closely related to the different way of degradation. The characterization of PBS/PLA blends after degradation showed that selective enzymatic degradation could not completely degrade PBS or PLA component. After degradation, the pores formed by proteinase K were more uniform and larger than those formed by cutinase. This work provides a new insight into the selective enzymatic degradation processes of the porous materials, which will be used in tissue engineering or oil-water separation in the future.
聚丁二酸丁二醇酯（PBS）和聚乳酸（PLA）以不同比例熔融共混，并分别用脂肪酶和蛋白酶 K 选择性降解。系统研究了选择性酶降解过程。通过扫描电子显微镜、傅里叶变换红外光谱、粉末 X 射线衍射和差示扫描量热法对降解的 PBS/PLA 共混物进行了分析。脂肪酶和蛋白酶 K 降解 PBS/PLA 共混物的失重结果表明，PLA 阻碍了脂肪酶催化的 PBS 降解，而在特定的 PBS/PLA 比例下，PBS 的添加加速了 PLA 的降解。降解后结晶度的变化与降解方式密切相关。降解后 PBS/PLA 共混物的表征表明，选择性酶降解不能完全降解 PBS 或 PLA 组分。降解后，蛋白酶 K 形成的孔比脂肪酶形成的孔更均匀、更大。这项工作为多孔材料的选择性酶降解过程提供了新的见解，未来将用于组织工程或油水分离。
