脂肪酶对不同分子量聚丁二酸丁二醇酯的酶解作用。
Enzymatic degradation of poly(butylene succinate) with different molecular weights by cutinase.
机构信息
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun 113001, China.
出版信息
Int J Biol Macromol. 2018 May;111:1040-1046. doi: 10.1016/j.ijbiomac.2018.01.107. Epub 2018 Jan 31.
Poly(butylene succinate) (PBS) films with different molecular weights were enzymatically degraded by cutinase. Changes in the properties of the films before and after enzymatic degradation were studied through scanning electron microscopy, differential scanning calorimetry, thermogravimetry, X-ray powder diffraction, proton nuclear magnetic resonance, and gel-permeation chromatography analysis. The weight loss of the films initially decreased and then increased with increasing molecular weight. Crystallinity was inversely proportional to weight loss and tended to decrease with prolonged degradation time. Crystalline and amorphous regions were simultaneously degraded. The thermal stability of PBS films decreased after enzymatic degradation. PBS was the main component of the enzymatically degraded polymers. The molecular weights of the films did not considerably change before and after degradation by cutinase.
聚丁二酸丁二醇酯（PBS）薄膜的分子量不同，用过氧化氢酶进行酶降解。通过扫描电子显微镜、差示扫描量热法、热重分析、X 射线粉末衍射、质子核磁共振和凝胶渗透色谱分析，研究了酶降解前后薄膜性能的变化。薄膜的失重最初随分子量的增加而减少，然后增加。结晶度与失重成反比，随降解时间的延长而趋于下降。结晶区和非晶区同时降解。PBS 薄膜的热稳定性在酶解后下降。PBS 是酶解聚合物的主要成分。用过氧化氢酶降解前后，薄膜的分子量没有明显变化。
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