Department of Cell Toxicology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
Department of Cell Toxicology, Helmholtz Centre for Environmental Research - UFZ, Permoserstr. 15, 04318 Leipzig, Germany.
Chemosphere. 2019 Apr;220:501-504. doi: 10.1016/j.chemosphere.2018.12.134. Epub 2018 Dec 20.
Passive equilibrium sampling using polymer samplers in lean tissue is one of the current challenges in assessing bioaccumulation and biomagnification due to the long time needed to reach equilibrium. Despite recent progress achieved by rolling pieces of intact fish fillet with sheets of silicone, there is still a need for a passive sampling method for homogenates that achieves equilibrium before tissue decay starts. In this work, a new approach for relocation of silicone passive samplers in homogenates of lean fish was established for three homogenates with lipid contents varying from 1.2% to 6.1%. Results showed that for 20 model hydrophobic organic compounds with log K between 3.9 and 7.8, equilibrium between the silicone and the tissue was achieved in less than 3 days at 4 °C. The concentrations in lipids obtained using passive equilibrium sampling and those from traditional total solvent extraction agreed well, within a factor of 1.3. This new procedure extends the use of passive samplers to homogenised fish tissues of low lipid content, which is highly relevant for environmental studies focused on bioaccumulation of contaminants.
在评估生物积累和生物放大时，由于达到平衡所需的时间较长，因此使用聚合物采样器在瘦肉组织中进行被动平衡采样是当前面临的挑战之一。尽管最近通过用硅酮片滚动完整鱼片的方法取得了进展，但仍需要一种能够在组织降解开始之前达到平衡的均质物的被动采样方法。在这项工作中，针对脂质含量从 1.2%到 6.1%不等的三种均质物，建立了一种新的在瘦肉鱼均质物中重新定位硅酮被动采样器的方法。结果表明，对于 log K 值在 3.9 到 7.8 之间的 20 种模型疏水性有机化合物，在 4°C 下，硅酮和组织之间在不到 3 天的时间内达到平衡。使用被动平衡采样获得的脂质浓度与传统的全溶剂萃取法获得的浓度非常吻合，误差在 1.3 以内。该新程序扩展了被动采样器在低脂质含量的均质化鱼组织中的使用，这对于专注于污染物生物积累的环境研究非常重要。
