Tamrakar Sonam, Fukami Katsuya, Parajuli Gopal Prasad, Shimizu Kuniyoshi
1 Faculty of Agriculture, Kyushu University , Fukuoka, Japan .
2 Material Management Center, Kyushu University , Fukuoka, Japan .
J Med Food. 2019 Feb;22(2):225-227. doi: 10.1089/jmf.2018.4267. Epub 2018 Dec 31.
In the present study, ethanol extracts of 90 wild mushroom samples from Nepal, and the pure compound hispidin, were screened for their ability to inhibit β-hexosaminidase release (BHR) from rat basophilic leukemia-2H3 cells. Simultaneously, the toxicity of the extracts toward the cells was also determined, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Samples belonging to the groups Hymenochaetales and Polyporales showed promising anti-allergic activity, with Phellinus adamantinus and Ganoderma lingzhi 3 allowing a mere 19.4% and 16.7% BHR, respectively, without any cell cytotoxicity. Moreover, the 50% inhibitory concentration (IC) values for Inonotus clemensiae and P. adamantinus were determined to be 51.24 and 50.65 μg/mL, respectively; whereas hispidin, the major bioactive compound in I. clemensiae showed an IC value of 82.47 μg/mL. These findings are crucial in underscoring the medicinal value of the wild mushrooms of Nepal, as a source of strong antiallergic agents.
在本研究中，对来自尼泊尔的90个野生蘑菇样本的乙醇提取物以及纯化合物漆斑菌素，进行了抑制大鼠嗜碱性白血病-2H3细胞释放β-己糖胺酶（BHR）能力的筛选。同时，还使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法确定了提取物对细胞的毒性。属于层菌纲和多孔菌目的样本显示出有前景的抗过敏活性，其中坚硬木层孔菌和灵芝3分别仅允许19.4%和16.7%的BHR，且没有任何细胞毒性。此外，确定克莱门斯纤孔菌和坚硬木层孔菌的50%抑制浓度（IC）值分别为51.24和50.65μg/mL；而克莱门斯纤孔菌中的主要生物活性化合物漆斑菌素的IC值为82.47μg/mL。这些发现对于强调尼泊尔野生蘑菇作为强效抗过敏剂来源的药用价值至关重要。
