Nanobiotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran; Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Int J Biol Macromol. 2019 Apr 15;127:27-38. doi: 10.1016/j.ijbiomac.2018.12.256. Epub 2018 Dec 28.
In this study, aqueous Schizophyllan (SPG) (1.5 w/v%) was mixed with aqueous polyvinyl alcohol (PVA) (10 w/v%) at various volume ratios and electrospun to prepare nanofibers. The fiber diameter was decreased by increasing the SPG content. A reliable linear relationship (p < 0.01) was established between the solution properties and fiber diameter. Contiguous, bead-free, and smooth fibers were obtained when the SPG/PVA ratios were 20:80, 30:70, and 40:60. FT-IR spectra, SEM images, tensile testing, swelling ratios, and water contact angle were utilized to characterize this glutaraldehyde (Glu) vapor cross-linked nanofibers in order to analyze the morphology, functional groups, mechanical attributes, hydrophilicity, and humidity of the nanofibers for skin tissue regeneration. Furthermore, the cell culture that was studied by the use of fibroblast (L929) cells showed that these SPG-based nanofibrous scaffolds could generate the improved cell adhesion. In conclusion, it was observed that SPG/PVA nanofiber mat in a volume ratio of 20:80 after 3 day was a suitable material for improving the wound healing, as it could increase cell proliferation and migration that possessed fiber diameter. The characteristics of this nanofiber were 267 nm, contact angle of 47.75°, good swelling behavior (289%), the ultimate strength of 6.513 MPa, Young modulus of 2.665 MPa, and cell viability of 150%.
在这项研究中，将水状裂裥菌素 (SPG)（1.5 w/v%）与水状聚乙烯醇 (PVA)（10 w/v%）以不同体积比混合，并进行电纺以制备纳米纤维。随着 SPG 含量的增加，纤维直径减小。溶液性质与纤维直径之间建立了可靠的线性关系（p < 0.01）。当 SPG/PVA 比例为 20:80、30:70 和 40:60 时，可获得连续、无珠、光滑的纤维。利用傅里叶变换红外光谱 (FT-IR)、扫描电子显微镜 (SEM) 图像、拉伸试验、溶胀比和水接触角对戊二醛 (Glu) 蒸气交联纳米纤维进行表征，以分析形貌、功能基团、机械性能、亲水性和纳米纤维的湿度，用于皮肤组织再生。此外，通过使用成纤维细胞 (L929) 细胞进行的细胞培养表明，这些基于 SPG 的纳米纤维支架可以提高细胞粘附。总之，观察到体积比为 20:80 的 SPG/PVA 纳米纤维垫在 3 天后是一种改善伤口愈合的合适材料，因为它可以增加具有纤维直径的细胞增殖和迁移。这种纳米纤维的特征为 267nm、接触角为 47.75°、良好的溶胀行为（289%）、极限强度为 6.513MPa、杨氏模量为 2.665MPa 和细胞活力为 150%。
