Physical Materials Science and Composite Materials Centre, National Research Tomsk Polytechnic University, Lenin Avenue, 30, 634050, Tomsk, Russian Federation.
Physical Materials Science and Composite Materials Centre, National Research Tomsk Polytechnic University, Lenin Avenue, 30, 634050, Tomsk, Russian Federation.
Colloids Surf B Biointerfaces. 2019 Apr 1;176:130-139. doi: 10.1016/j.colsurfb.2018.12.047. Epub 2018 Dec 18.
In the present study, biocomposites based on 3D porous additively manufactured Ti6Al4V (Ti64) scaffolds modified with biocompatible calcium phosphate nanoparticles (CaPNPs) were investigated. Ti64 scaffolds were manufactured via electron beam melting technology using an Arcam machine. Electrophoretic deposition was used to modify the scaffolds with CaPNPs, which were synthesized by precipitation in the presence of polyethyleneimine (PEI). Dynamic light scattering revealed that the CaP/PEI nanoparticles had an average size of 46 ± 18 nm and a zeta potential of +22 ± 9 mV. Scanning electron microscopy (SEM) revealed that the obtained spherical CaPNPs had an average diameter of approximately 90 nm. The titanium-based scaffolds coated with CaPNPs exhibited improved hydrophilic surface properties, with a water contact angle below 5°. Cultivation of human mesenchymal stem cells (hMSCs) on the CaPNPs-coated Ti64 scaffolds indicated that the improved hydrophilicity was beneficial for the attachment and growth of cells in vitro. The Ti6Al4V/CaPNPs scaffold supported an increase in the alkaline phosphatase (ALP) activity of cells. In addition to the favourable cell proliferation and differentiation, Ti6Al4V/CaPNPs scaffolds displayed increased mineralization compared to non-coated Ti6Al4V scaffolds. Thus, the developed composite 3D scaffolds of Ti6Al4V functionalized with CaPNPs are promising materials for different applications related to bone repair.
在本研究中，研究了基于 3D 多孔增材制造 Ti6Al4V（Ti64）支架的生物复合材料，该支架经过生物相容性钙磷纳米粒子（CaPNPs）修饰。Ti64 支架通过电子束熔化技术使用 Arcam 机器制造。通过电泳沉积将 CaPNPs 修饰到支架上，CaPNPs 通过在聚乙烯亚胺（PEI）存在下沉淀合成。动态光散射表明，CaP/PEI 纳米颗粒的平均粒径为 46±18nm，zeta 电位为+22±9mV。扫描电子显微镜（SEM）显示，所得的球形 CaPNPs 的平均直径约为 90nm。涂有 CaPNPs 的钛基支架表现出改善的亲水表面性能，水接触角低于 5°。人骨髓间充质干细胞（hMSCs）在 CaPNPs 涂覆的 Ti64 支架上的培养表明，改善的亲水性有利于细胞的体外附着和生长。Ti6Al4V/CaPNPs 支架支持细胞碱性磷酸酶（ALP）活性的增加。除了有利的细胞增殖和分化外，Ti6Al4V/CaPNPs 支架与未涂覆的 Ti6Al4V 支架相比，矿化程度增加。因此，用 CaPNPs 功能化的 Ti6Al4V 开发的这种复合 3D 支架是与骨修复相关的不同应用的有前途的材料。
