Ramage Gordon, O'Donnell Lindsay, Sherry Leighann, Culshaw Shauna, Bagg Jeremy, Czesnikiewicz-Guzik Marta, Brown Clare, McKenzie Debbie, Cross Laura, MacInnes Andrew, Bradshaw David, Varghese Roshan, Gomez Pereira Paola, Jose Anto, Sanyal Susmita, Robertson Douglas
School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
GSK Consumer Healthcare, Weybridge, UK.
J Oral Microbiol. 2018 Oct 29;11(1):1538437. doi: 10.1080/20002297.2018.1538437. eCollection 2019.
: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. : (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. : The model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both and studies showed that denture plaque biofilm composition were affected by different treatment arms. : This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Clinicaltrials.gov registration: NCT02780661.
目前缺乏关于如何恰当管理义齿菌斑的有力科学和临床证据。这项分为两部分的研究：（i）建立了义齿菌斑清除模型，（ii）在一项随机临床试验中评估了这些方法的有效性。：（i）利用近期微生物组分析中的优势微生物属建立了一个复杂的义齿菌斑模型。在聚甲基丙烯酸甲酯上形成的生物膜每天用湿牙刷刷洗，然后每天处理5天，或仅在第1天和第5天用Polident®义齿清洁片处理（浸泡3分钟）。进行了定量和定性的微生物学评估。（ii）对全口上颌义齿佩戴者进行了一项研究者盲法、随机、交叉研究（n = 19）。参与者要么每天浸泡义齿15分钟，持续7天，要么仅在第7天使用Corega®义齿清洁片浸泡，然后刷牙。义齿菌斑微生物学评估使用无菌滤纸圆盘。：模型显示，与每天刷牙的间歇性义齿清洁相比，使用义齿清洁片并每天刷牙能显著减少微生物数量（p < 0.001）。该研究的临床部分显示，义齿菌斑微生物数量在每日治疗组与每周治疗组之间有统计学意义的显著减少（需氧菌p = 0.0144）。模型研究和临床研究均表明，不同治疗组会影响义齿菌斑生物膜的组成。：这项研究表明，每日义齿清洁方案优于间歇性义齿清洁，并且清洁方案可导致义齿菌斑组成发生变化。Clinicaltrials.gov注册号：NCT02780661。
