College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Molecules. 2018 Dec 31;24(1):136. doi: 10.3390/molecules24010136.
Volatile sulfur compounds gradually develop in after hot-air drying, and many genes are involved in the generation of these sulfur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR). In this study, the expression profile of 17 candidate genes was assessed in under treatment at 50 °C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper. Results indicated that the two optimal reference genes for mycelium and fruiting body were and as well as and , respectively. Additionally, and were found to be the two most stable reference genes across the mycelium and fruiting body set. Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in .
在热空气干燥后，挥发性硫化合物逐渐在 中产生，许多基因参与了这些硫化合物的生成。在通过实时定量聚合酶链反应（qRT-PCR）分析其表达时，参考基因的表达稳定性在特定的实验处理中可能会有所不同。在这项研究中，评估了 17 个候选基因在 50°C 下处理 0、1、2 和 3 小时后的表达谱，并通过三个统计算法（geNorm、NormFinder 和 BestKeeper）分析了每个参考基因的表达稳定性。结果表明，对于菌丝体和子实体，最佳的两个参考基因分别为 和 以及 。此外， 和 被发现是菌丝体和子实体中最稳定的两个参考基因。我们的研究结果将为进一步研究硫化合物代谢基因在 中的作用提供遗传基础。
