Won Sun, Huh Yoon-Hyuk, Cho Lee-Ra, Lee Hee-Su, Byon Eung-Sun, Park Chan-Jin
1Department of Prosthodontics and Research Institute of Oral Science, College of Dentistry, Gangneung-Wonju National University, Jukheongil 7, Gangneung, Gangwon-do 26403 Republic of Korea.
2Department of Anatomy and Research Institute of Oral Science, College of Dentistry, Gangneung-Wonju National University, Gangwon-do, 26403 Republic of Korea.
Tissue Eng Regen Med. 2017 Mar 8;14(2):123-131. doi: 10.1007/s13770-017-0028-3. eCollection 2017 Apr.
Surface characteristics and cellular response to titanium surfaces that had been implanted with calcium and magnesium ions using plasma immersion ion implantation and deposition (PIIID) were evaluated. Three different titanium surfaces were analyzed: a resorbable blast media (RBM) surface (blasted with hydroxyapatite grit), a calcium ion-implanted surface, and a magnesium ion-implanted surface. The surface characteristics were investigated by scanning electron microscopy (SEM), surface roughness testing, X-ray diffraction (XRD), and Auger electron spectroscopy (AES). Human bone marrow derived mesenchymal stem cells were cultured on the 3 different surfaces. Initial cell attachment was evaluated by SEM, and cell proliferation was determined using MTT assay. Real-time polymerase chain reaction (PCR) was used to quantify osteoblastic gene expression (i.e., genes encoding RUNX2, type I collagen, alkaline phosphatase, and osteocalcin). Surface analysis did not reveal any changes in surface topography after ion implantation. AES revealed that magnesium ions were present in deeper layers than calcium ions. The calcium ion- and magnesium ion-implanted surfaces showed greater initial cell attachment. Investigation of cell proliferation revealed no significant difference among the groups. After 6 days of cultivation, the expression of RUNX2 was higher in the magnesium ion-implanted surface and the expression of osteocalcin was lower in the calcium ion-implanted surface. In conclusion, ion implantation using the PIIID technique changed the surface chemistry without changing the topography. Calcium ion- and magnesium ion-implanted surfaces showed greater initial cellular attachment.
评估了通过等离子体浸没离子注入和沉积（PIIID）植入钙和镁离子的钛表面的表面特性及细胞反应。分析了三种不同的钛表面：可吸收喷砂介质（RBM）表面（用羟基磷灰石颗粒喷砂）、钙离子植入表面和镁离子植入表面。通过扫描电子显微镜（SEM）、表面粗糙度测试、X射线衍射（XRD）和俄歇电子能谱（AES）研究表面特性。将人骨髓间充质干细胞培养在这三种不同表面上。通过SEM评估初始细胞附着情况，并使用MTT法测定细胞增殖。采用实时聚合酶链反应（PCR）定量成骨细胞基因表达（即编码RUNX2、I型胶原蛋白、碱性磷酸酶和骨钙素的基因）。表面分析未发现离子注入后表面形貌有任何变化。AES显示镁离子存在于比钙离子更深的层中。钙离子和镁离子植入表面显示出更大的初始细胞附着。细胞增殖研究表明各组之间无显著差异。培养6天后，RUNX2在镁离子植入表面的表达较高，而骨钙素在钙离子植入表面的表达较低。总之，使用PIIID技术进行离子注入改变了表面化学性质，而未改变形貌。钙离子和镁离子植入表面显示出更大的初始细胞附着。
