Kim Seunghyun, Ahn Taeho, Han Myung-Ho, Bae Chunsik, Oh Daniel S
1College of Veterinary Medicine, Chonnam National University, 77 Yongbong-ro, Gwangju, 61186 Republic of Korea.
2Department of Chemical Engineering, Kyungil University, 50 Gamasil-gil, Gyeongsan, 38428 Republic of Korea.
Tissue Eng Regen Med. 2018 Jul 13;15(4):503-510. doi: 10.1007/s13770-018-0142-x. eCollection 2018 Aug.
Recruitment and homing cells into graft materials from host tissue is crucial for bone regeneration.
Highly porous, multi-level structural, hydroxyapatite bone void filler (HA-BVF) have been investigated to restore critical size bone defects. The aim was to investigate a feasibility of bone regeneration of synthetic HA-BVF compared to commercial xenograft (Bio-Oss). HA-BVF of 0.7 mm in average diameter was prepared via template coating method. Groups of animals (n = 6) were divided into two with normal (Sham) or induced osteoporotic conditions (Ovx). Subsequently, subdivided into three treated with HA-BVF as an experiment or Bio-Oss as a positive control or no treatment as a negative control (defect). The new bone formation was analyzed by micro-CT and histology.
At 4 weeks post-surgery, new bone formation was initiated from all groups. At 8 weeks post-surgery, new bone formation in the HA-BVF groups was greater than Bio-Oss groups. Extraordinarily greater bone regeneration within the Ovx-HA group than Sham-Bio-Oss or Ovx-Bio-Oss group (< 0.05).
This study suggests that the immediate wicking property of HA-BVF from host tissue activates a natural healing cascade without the addition of exogeneous factors or progenitor cells. HA-BVF may be an effective alternative for repairing bone defects under both normal and osteoporotic bone conditions.
宿主组织中的细胞募集并归巢到移植材料中对骨再生至关重要。
已对高度多孔、多级结构的羟基磷灰石骨缺损填充材料（HA-BVF）进行研究，以修复临界尺寸的骨缺损。目的是研究合成的HA-BVF与商业异种移植物（Bio-Oss）相比骨再生的可行性。通过模板涂层法制备平均直径为0.7毫米的HA-BVF。将动物组（n = 6）分为正常（假手术）或诱导骨质疏松状态（卵巢切除）两组。随后，再细分为三组，分别用HA-BVF进行实验治疗、用Bio-Oss作为阳性对照或不进行治疗作为阴性对照（缺损）。通过显微CT和组织学分析新骨形成情况。
术后4周，所有组均开始有新骨形成。术后8周，HA-BVF组的新骨形成大于Bio-Oss组。卵巢切除-HA组内的骨再生明显大于假手术-Bio-Oss组或卵巢切除-Bio-Oss组（<0.05）。
本研究表明，HA-BVF从宿主组织的即时吸湿特性可激活自然愈合级联反应，而无需添加外源性因子或祖细胞。HA-BVF可能是在正常和骨质疏松骨条件下修复骨缺损的有效替代物。
