State Key Laboratory of Crop Genetics of Disease Resistance and Disease Control, State Key Laboratory of Hybrid Rice, Key Laboratory of Major Crop Diseases & Collaborative Innovation Center for Hybrid Rice in Yangtze River Basin, Rice Research Institute, Sichuan Agricultural University, Sichuan 611130, China.
Department of Plant Pathology, University of California, Davis, CA 95616, USA.
J Genet Genomics. 2018 Dec 20;45(12):663-672. doi: 10.1016/j.jgg.2018.10.007. Epub 2018 Dec 31.
Map-based cloning of plant disease resistance (R) genes is time-consuming. Here, we reported the isolation of blast R gene Pid4 using comparative transcriptomic profiling and genome-wide sequence analysis. Pid4 encodes a coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) protein and is constitutively expressed at diverse developmental stages in the rice variety Digu. The Pid4 protein is localized in both the nucleus and cytoplasm. Introduction of Pid4 into susceptible rice cultivars confers race-specific resistance to leaf and neck blast. Amino acid sequence comparison and blast resistance spectrum tests showed that Pid4 is a novel R gene, different from the previously reported R genes located in the same gene cluster. A Pid4 Indel marker was developed to facilitate the identification of Pid4 in different rice varieties. We demonstrated that a plant R gene can be quickly isolated using transcriptomic profiling coupled with genome-wide sequence analysis.
基于图谱的植物抗病（R）基因克隆耗时耗力。本研究通过比较转录组分析和全基因组序列分析，报告了使用该方法克隆水稻稻瘟病 R 基因 Pid4 的过程。Pid4 编码一个卷曲螺旋核苷酸结合位点富含亮氨酸重复（CC-NBS-LRR）蛋白，在供试品种 Digu 的不同发育阶段持续表达。Pid4 蛋白定位于细胞核和细胞质。将 Pid4 导入感病水稻品种，可赋予其对叶片和穗颈瘟的专化抗性。氨基酸序列比较和抗病谱测试表明，Pid4 是一个新的 R 基因，与位于同一基因簇的先前报道的 R 基因不同。开发了 Pid4 插入缺失标记，以方便在不同水稻品种中鉴定 Pid4。本研究证明，通过转录组分析与全基因组序列分析相结合，可以快速分离植物 R 基因。
