Polymers Laboratory, Instituto Potosino de Investigación Científica y Tecnológica A.C. San Luís Potosí, 78216 SLP, Mexico.
Polymers Laboratory, Instituto Potosino de Investigación Científica y Tecnológica A.C. San Luís Potosí, 78216 SLP, Mexico.
Mater Sci Eng C Mater Biol Appl. 2019 Mar;96:798-806. doi: 10.1016/j.msec.2018.12.003. Epub 2018 Dec 4.
The engineering of bone tissues represents an area of opportunity for the development of new polymeric compounds. In this context, the objective of this work is the generation and evaluation in vitro of supports obtained from mixtures of starch with poly (lactic acid) (PLA), treated with arginine-glycine-aspartic acid peptides (RGD). For this, non-woven fibers of PLA with different starch content (0.0, 2.5, 5.0 and 10.0%wt) were obtained using the electrospinning technique. Then the physical absorption of RGD was carried out, with the aim of increasing the cellular adhesion of the polymeric material. Subsequently, in vitro biocompatibility tests were performed, and viability (LIFE/DEAD), proliferation (MTS assay) and cell adhesion were carried out with osteoblasts incubated for 48 h. Regarding biocompatibility results, only viable cells were found for all the compositions, and the biocompatibility of the materials was validated by the morphological analysis of the cultured cells, where extended cells were observed. Proliferation assays show that osteoblasts proliferate better on the surfaces of PLA and PLA with 5.0% starch scaffolds. Therefore, it is concluded that the scaffolds obtained by electrospinning of PLA with starch and functionalized with RGD are promising for its use in the regeneration of bone tissue.
骨组织工程是开发新型聚合物化合物的一个机会领域。在这种情况下，这项工作的目的是生成和体外评估由淀粉与聚乳酸（PLA）混合物制成的支架，并用精氨酸-甘氨酸-天冬氨酸肽（RGD）进行处理。为此，使用静电纺丝技术获得了不同淀粉含量（0.0、2.5、5.0 和 10.0%wt）的 PLA 无纺纤维。然后进行 RGD 的物理吸收，目的是提高聚合物材料的细胞黏附性。随后，进行了体外生物相容性测试，用成骨细胞孵育 48 h 进行了活力（LIFE/DEAD）、增殖（MTS 测定）和细胞黏附测试。关于生物相容性结果，所有组合物中仅发现有活力的细胞，并且通过培养细胞的形态分析验证了材料的生物相容性，在该分析中观察到了扩展的细胞。增殖测定表明，成骨细胞在 PLA 和含有 5.0%淀粉支架的 PLA 表面上增殖更好。因此，可以得出结论，用 RGD 功能化的 PLA 和淀粉静电纺丝获得的支架有望用于骨组织再生。
