基于聚苯胺的材料作为一种新型 SPE 吸附剂，用于在生物碱色谱分析前对白屈菜提取物进行预处理。

Polyaniline based material as a new SPE sorbent for pre-treatment of Chelidonium majus extracts before chromatographic analysis of alkaloids.

机构信息

Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.

Department of Plant Physiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland.

出版信息

Talanta. 2019 Mar 1;194:32-37. doi: 10.1016/j.talanta.2018.10.009. Epub 2018 Oct 10.

DOI:10.1016/j.talanta.2018.10.009

PMID:30609537

Abstract

In the present study, deprotonated and protonated polyaniline based sorbent obtained by in situ polymerization of aniline on silica (Si-PANI) was applied for SPE pretreatment of Chelidonium majus extracts before determination of benzophenanthridine, protoberberine and protopine alkaloids. The experimentally optimized conditions, such as protonated polyaniline impregnated with methanol as a sorbent, 2 mL of water and methanol mixture (1/1, v/v) as a washing solution, and 5 mL of 0.1 M methanolic solution of ammonia as an elution solvent, yielded the highest values of analyte recovery (above 96.5%) with simultaneous removal of undesirable plant matrix. Virtually modeled structure of polyaniline revealed possible occurrence of the mixed mode binding mechanism, based both on π-π and ion interactions on protonated form of polyaniline. Moreover, lack of significant changes of the polyaniline film quality assessed by Raman spectroscopy after series of ten experiments proved the reusability of the sorbent.

摘要

在本研究中，通过苯胺在硅胶（Si-PANI）上的原位聚合获得的去质子化和质子化聚苯胺基吸附剂用于Chelidonium majus 提取物的 SPE 预处理，然后测定苯并菲啶、原小檗碱和原阿片碱类生物碱。通过实验优化的条件，例如用甲醇浸渍的质子化聚苯胺作为吸附剂、2 mL 水和甲醇混合物（1/1，v/v）作为洗涤溶液、5 mL 0.1 M 氨甲醇溶液作为洗脱溶剂，可获得最高的分析物回收率（高于 96.5%），同时去除不需要的植物基质。通过对聚苯胺的虚拟建模结构研究发现，基于质子化聚苯胺的π-π 和离子相互作用，可能发生混合模式结合机制。此外，拉曼光谱评估的一系列十次实验后聚苯胺膜质量没有明显变化，证明了吸附剂的可重复使用性。