长链非编码RNA DANCR通过抑制p21表达促进非小细胞肺癌进展。

Long non-coding RNA DANCR promotes the progression of non-small-cell lung cancer by inhibiting p21 expression.

作者信息

Guo Lanfang, Gu Jianmei, Hou Sinan, Liu Dabiao, Zhou Mengjie, Hua Tengjiang, Zhang Jinye, Ge Zhijun, Xu Jing

机构信息

Department of Clinical Laboratory Medicine, The Fourth People's Hospital of Zhenjiang, Zhenjiang, Jiangsu 212013, China.

Departmemt of Clinical Laboratory Medicine, Nantong Tumor Hospital, Nantong, Jiangsu 226361, China.

出版信息

Onco Targets Ther. 2018 Dec 21;12:135-146. doi: 10.2147/OTT.S186607. eCollection 2019.

BACKGROUND

Long non-coding RNAs (lncRNAs) play important roles in human cancers. However, the functional roles of lncRNAs in non-small-cell lung cancer (NSCLC) and the underlying mechanisms are not well understood.

METHODS

We examined the expression of lncRNA DANCR in NSCLC by qRT-PCR and explored its biological roles in NSCLC progression by cell and molecular biology studies.

RESULTS

DANCR expression level was increased in human NSCLC. The knockdown of DANCR inhibited NSCLC cell proliferation by inducing cell apoptosis and cell cycle arrest. In addition, DANCR knockdown suppressed NSCLC cell migration and invasion via inhibition of epithelial-mesenchymal transition (EMT). On the contrary, DANCR overexpression had the opposite effects. DANCR knockdown inhibited EZH-2-mediated epigenetic silencing of p21 promoter and increased p21 expression. Moreover, DANCR knockdown inhibited NSCLC cell proliferation, migration, and invasion in a p21-dependent manner.

CONCLUSION

DANCR plays oncogenic roles in NSCLC and may provide a novel biomarker for NSCLC diagnosis and prognosis.

背景

长链非编码RNA（lncRNAs）在人类癌症中发挥重要作用。然而，lncRNAs在非小细胞肺癌（NSCLC）中的功能作用及潜在机制尚未完全明确。

方法

我们通过qRT-PCR检测lncRNA DANCR在NSCLC中的表达，并通过细胞和分子生物学研究探索其在NSCLC进展中的生物学作用。

结果

DANCR在人类NSCLC中的表达水平升高。敲低DANCR可通过诱导细胞凋亡和细胞周期阻滞抑制NSCLC细胞增殖。此外，敲低DANCR通过抑制上皮-间质转化（EMT）抑制NSCLC细胞迁移和侵袭。相反，DANCR过表达则产生相反的效果。敲低DANCR可抑制EZH-2介导的p21启动子表观遗传沉默并增加p21表达。此外，敲低DANCR以p21依赖的方式抑制NSCLC细胞增殖、迁移和侵袭。