Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea; Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Advanced Biomass R&D Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Bioresour Technol. 2019 Mar;276:110-118. doi: 10.1016/j.biortech.2018.12.111. Epub 2018 Dec 31.
In this work, the two-stage cultivation of Chlorella sp. HS2 for enhancing the lipid productivity was optimized by adjusting the duration of nitrogen-replete (N+) and -deplete (N-) stages within a 9 day period using urea as nitrogen source. The highest lipid content of 36.7% and productivity of 216.9 mg L d were obtained under five days of N+ followed by four days of N- conditions. Replenishing phosphorus and other nutrients (N-P+O+) at the beginning of the nutrient-starvation resulted in 1.55 and 1.68-folds improvement in lipid productivities compared to the single stage and zero nutrient controls (N-P-O-), respectively. The estimated biodiesel properties based on the fatty acid profiles met all criteria of international standards. The findings of this study indicate that properly adjusting the period of nitrogen availability as well as the presence of other nutrients is highly important in order to maximize the biofuel productivity in two-stage microalgal cultivation.
在这项工作中，通过使用尿素作为氮源，在 9 天的时间内调整富氮 (N+) 和贫氮 (N-) 阶段的持续时间，优化了两轮培养小球藻 HS2 以提高油脂生产力。在 N+ 阶段持续 5 天，随后 N- 阶段持续 4 天的条件下，获得了最高的 36.7%的脂质含量和 216.9mg/L/d 的生产力。在营养饥饿开始时补充磷和其他营养物质 (N-P+O+)，与单阶段和零营养对照 (N-P-O-) 相比，脂质生产力分别提高了 1.55 和 1.68 倍。根据脂肪酸谱估计的生物柴油特性符合国际标准的所有标准。本研究结果表明，为了最大限度地提高两轮微藻培养中的生物燃料生产力，适当调整氮供应的时间以及其他营养物质的存在非常重要。
