Romero C H, Purchase H G, Frank F R, Chang T S
Avian Dis. 1978 Jan-Mar;22(1):53-60.
Bursa-derived stem cells in the spleens of chickens treated with the androgen analog mibolerone or with cyclophosphamide and in the spleens of untreated controls were examined by a transplantation technique. Spleen cells from syngeneic donors 2-20 weeks old were transferred to syngeneic cyclophosphamide-treated recipients, and the immunocompetence of the recipients was tested 5 weeks after the transfer by antigenic stimuli with sheep erythrocytes and Brucella abortus. Spleens of mibolerone-treated chickens failed to reconstitute the immunologic capability of cyclophosphamide-treated recipients in a manner similar to spleen cells from normal donors. Spleens of cyclophosphamide-treated donors did not contain stem cells. Stem cells were detected in the spleens of untreated controls as young as 2 weeks old; activity was highest after the 13th week of age.
采用移植技术对用雄激素类似物米勃酮或环磷酰胺处理过的鸡脾脏以及未处理的对照鸡脾脏中源自滑囊的干细胞进行了检测。将2至20周龄同基因供体的脾细胞转移至经环磷酰胺处理的同基因受体，转移5周后通过用绵羊红细胞和流产布鲁氏菌进行抗原刺激来检测受体的免疫能力。用米勃酮处理过的鸡的脾脏无法像正常供体的脾细胞那样重建经环磷酰胺处理的受体的免疫能力。经环磷酰胺处理的供体的脾脏不含干细胞。在2周龄的未处理对照鸡脾脏中就检测到了干细胞；活性在13周龄后最高。
