Bhanushali J K, Murthy K K, Ragland W L
Immunopharmacology. 1985 Oct;10(2):99-110. doi: 10.1016/0162-3109(85)90035-9.
The effects of various doses of mibolerone, an analog of testosterone, in the development of the bursa of Fabricius and the humoral immune system were examined by injecting 0.01, 0.1, 1, 10 or 100 micrograms mibolerone into the chorio-allantoic cavity of 12-day-old chick embryos. Treatment with 0.01 or 0.1 micrograms of mibolerone caused slight or no bursal atrophy, 1 microgram caused substantial bursal atrophy, and 10 or 100 micrograms caused severe bursal atrophy at the time of hatching and at 6 weeks of age. When mibolerone-treated birds were challenged with sheep red blood cells and killed Brucella abortus at 4 and 5 weeks of age, birds treated with 10 or 100 micrograms of mibolerone had suppressed humoral responses to sheep erythrocytes and B. abortus whereas birds treated with 0.01 and 0.1 micrograms of mibolerone had normal humoral immune responses. Birds treated with 1 microgram mibolerone had normal humoral immune responses to sheep erythrocytes and B. abortus (except for low IgG responses to B. abortus) despite the fact that the birds had substantially atrophic bursae during their embryonic and post-embryonic development. These results are discussed in relation to an earlier claim that mibolerone given in microgram quantities in feed during the post-hatch period causes rapid development of the humoral immune system.
通过向12日龄鸡胚的尿囊腔注射0.01、0.1、1、10或100微克的米勃酮（一种睾酮类似物），研究了不同剂量的米勃酮对法氏囊和体液免疫系统发育的影响。用0.01或0.1微克米勃酮处理导致轻微或无法氏囊萎缩，1微克导致明显的法氏囊萎缩，10或100微克在孵化时和6周龄时导致严重的法氏囊萎缩。当用米勃酮处理的鸡在4和5周龄时用绵羊红细胞和灭活的流产布鲁氏菌进行攻击时，用10或100微克米勃酮处理的鸡对绵羊红细胞和流产布鲁氏菌的体液反应受到抑制，而用0.01和0.1微克米勃酮处理的鸡具有正常的体液免疫反应。尽管在用1微克米勃酮处理的鸡在胚胎期和胚后发育期间法氏囊明显萎缩，但它们对绵羊红细胞和流产布鲁氏菌仍具有正常的体液免疫反应（除了对流产布鲁氏菌的低IgG反应）。这些结果与早期的一项说法相关进行了讨论，该说法称在孵化后期间在饲料中给予微克量的米勃酮会导致体液免疫系统快速发育。
