Bhanushali J K, Ragland W L
Vet Immunol Immunopathol. 1985 Nov;10(2-3):189-203. doi: 10.1016/0165-2427(85)90046-7.
The bursa anlage failed to develop in chicken embryos injected with 5 micrograms mibolerone on the 5th day of embryonation. Humoral immune responses in treated birds were studied by challenging them with sheep red blood cells (SRBC) and killed Brucella abortus (B. abortus) at 4, 5, 6 and 7 weeks of age. Control birds responded with IgM and IgG titers to SRBC and B. abortus, whereas mibolerone-treated birds completely failed to respond to B. abortus and their responses to SRBC consisted of only IgM agglutinins. Mibolerone-treated birds lacked natural agglutinins to rabbit red blood cells. The total concentration of plasma IgG, measured by radial immunodiffusion, was diminished whereas the IgM level was not influenced by the mibolerone treatment. Mibolerone-treated birds lacked bursal cell specific antigens in the peripheral blood leucocytes (PBL) but they still had Ig-positive fluorescing cells which were fewer in number than those of controls. Splenomegaly resulting from graft vs. host reactions induced in chick embryos by PBL from mibolerone-treated birds was similar to that in control birds, indicating normal alloreactive T cells. These results suggest that IgM responses observed in mibolerone-treated birds are produced by cells from an extrabursal site, and that mibolerone can be used effectively to chemically bursectomize chickens.
在胚胎发育第5天注射5微克米勃龙的鸡胚中，法氏囊原基未能发育。通过在4、5、6和7周龄时用绵羊红细胞（SRBC）和灭活的流产布鲁氏菌（B. abortus）对处理过的鸡进行攻毒，研究其体液免疫反应。对照鸡对SRBC和B. abortus产生IgM和IgG抗体滴度，而米勃龙处理的鸡对B. abortus完全无反应，它们对SRBC的反应仅由IgM凝集素组成。米勃龙处理的鸡缺乏对兔红细胞的天然凝集素。通过放射免疫扩散法测定，血浆IgG的总浓度降低，而IgM水平不受米勃龙处理的影响。米勃龙处理的鸡在外周血白细胞（PBL）中缺乏法氏囊细胞特异性抗原，但它们仍有Ig阳性荧光细胞，其数量比对照鸡少。米勃龙处理的鸡的PBL在鸡胚中诱导的移植物抗宿主反应导致的脾肿大与对照鸡相似，表明同种异体反应性T细胞正常。这些结果表明，米勃龙处理的鸡中观察到的IgM反应是由法氏囊外部位的细胞产生的，并且米勃龙可有效地用于化学去法氏囊鸡。
