Sheir Sherin K, Galal-Khallaf Asmaa, Mohamed Azza Hassan, Mohammed-Geba Khaled
Invertebrates Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
Heliyon. 2018 Dec 29;4(12):e01110. doi: 10.1016/j.heliyon.2018.e01110. eCollection 2018 Dec.
is a native species to the New World, and became an exotic species to most parts of the world. The Egyptian hypersaline, continental Qaroun Lake (Fayoum Governorate, Middle of Egypt) is subjected to a gradually increasing salinity rates that approximate or exceed these of seawater. populations there are known to be parthenogenetic. Yet, these populations started to exhibit abnormal morphologies. Therefore, Qaroun Lake samples of were subjected to several morphological, biometric, and molecular phylogenetic analyses for accurate species identification and phylogeographic origin approximation. These analyses revealed the existence of the alien sexual species of brine shrimp in Qaroun Lake. The characteristics of the subspherical frontal knob with several spines on the top, ovisac lateral triangular lobe on both sides and its projection together with the biometrics confirmed this species morphotype. DNA barcoding and other molecular analyses based on PCR-based amplification and sequencing of the barcode region of the cytochrome oxidase subunit I gene (COI) exhibited that all the collected samples belong to five haplotypes. Egyptian COI sequences phylogeny and pairwise distances analysis exhibited closer proximity to Latin American strains than to the Northern American ones. presence may be ascribed to the migratory birds present in Qaroun Lake protectorate, since no marine aquaculture activity in Qaroun Lake is known. Therefore, and for the best of our knowledge, this is the first record of the invasive in Egypt.
是新世界的本土物种，在世界大部分地区成为外来物种。埃及高盐度的内陆卡伦湖（法尤姆省，埃及中部）的盐度逐渐上升，接近或超过海水盐度。那里的种群已知是孤雌生殖的。然而，这些种群开始出现异常形态。因此，对卡伦湖的样本进行了多项形态学、生物测量学和分子系统发育分析，以进行准确的物种鉴定和系统地理起源近似分析。这些分析揭示了卡伦湖中存在卤虫的外来有性物种。顶部有几个刺的亚球形额瘤、两侧的卵囊侧三角叶及其突起的特征以及生物测量结果证实了该物种的形态类型。基于细胞色素氧化酶亚基I基因（COI）条形码区域的PCR扩增和测序的DNA条形码和其他分子分析表明，所有采集的样本属于五个单倍型。埃及卤虫COI序列系统发育和成对距离分析表明，与拉丁美洲菌株的亲缘关系比与北美菌株更近。其存在可能归因于卡伦湖保护区的候鸟，因为卡伦湖没有已知的海水养殖活动。因此，据我们所知，这是埃及首次记录到入侵卤虫。
