梅毒螺旋体强毒株与雅司螺旋体在各种哺乳动物细胞梯度培养中的比较行为。
Comparative behavior of virulent strains of Treponema pallidum and Treponema pertenue in gradient cultures of various mammalian cells.
作者信息
Fieldsteel A H, Stout J G, Becker F A
出版信息
Infect Immun. 1979 May;24(2):337-45. doi: 10.1128/iai.24.2.337-345.1979.
Two strains of virulent Treponema pallidum and two of virulent T. pertenue were investigated for their ability to attach to and survive in gradient cultures of five different mammalian cells under aerobic conditions. The strains of T. pallidum studied were the high-rabbit-passage Nichols and the low-rabbit-passage KKJ. The former was known to readily attach to cottontail rabbit epithelial cells (Sf1Ep) and to survive in the virulent state for up to 21 days. We therefore compared attachment of the other virulent treponemes with that of T. pallidum (Nichols). The KKJ strain of T. pallidum behaved in a fashion similar to T. pallidum (Nichols) in all of the cultures. Both strains exhibited preferential attachment to cells of Sf1Ep and those derived from the ear of a nude athymic (nu/nu) mouse. In these cultures, we observed a consistent three- to fivefold increase in attached treponemes up to 12 days after initial inoculation. The strains of T. pertenue were the human-derived Gauthier and cynocephalus-derived FB. These two strains of T. pertenue also attached to cells of all five types of cultures, but in smaller numbers than were seen with T. pallidum and equally to all of the cultures. Neither preferential attachment to Sf1Ep and nude mouse ear cells nor increased attachment with time was seen.
对两株毒力强的梅毒螺旋体和两株毒力强的雅司螺旋体进行了研究，以考察它们在有氧条件下附着于五种不同哺乳动物细胞的梯度培养物并在其中存活的能力。所研究的梅毒螺旋体菌株是高兔传代的Nichols株和低兔传代的KKJ株。前者已知能轻易附着于棉尾兔上皮细胞（Sf1Ep），并在毒力状态下存活长达21天。因此，我们将其他毒力强的密螺旋体与梅毒螺旋体（Nichols株）的附着情况进行了比较。梅毒螺旋体的KKJ株在所有培养物中的表现与梅毒螺旋体（Nichols株）相似。两株菌均表现出对Sf1Ep细胞和源自无胸腺裸鼠（nu/nu）耳部的细胞的优先附着。在这些培养物中，我们观察到在初次接种后长达12天，附着的密螺旋体数量持续增加三到五倍。雅司螺旋体菌株是源自人类的Gauthier株和源自猕猴的FB株。这两株雅司螺旋体也附着于所有五种培养类型的细胞，但数量比梅毒螺旋体少，且对所有培养物的附着情况相同。未观察到对Sf1Ep细胞和裸鼠耳部细胞的优先附着，也未观察到随时间附着增加的情况。
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