Ávila-Fernández Ángela, Galicia-Lagunas Nancy, Rodríguez-Alegría María E, Olvera Clarita, López-Munguía Agustín
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, Morelos 62250, Mexico.
Food Chem. 2011 Nov 15;129(2):380-386. doi: 10.1016/j.foodchem.2011.04.088. Epub 2011 May 4.
A controlled acid thermal hydrolysis process of fructans from agave (Agave tequilana Weber var. azul) was designed to produce a mixture of functional prebiotic fructooligosaccharides and sweetening power. Despite its highly branched structure, first-order kinetic behaviour with respect to substrate concentration was found with an activation energy of 95kJ/mol, similar to the value found for other linear fructans such as chicory inulin. Fructose equivalent (FE) an analogous parameter to dextrose equivalent (DE) used in the starch industry was introduced to characterise fructan hydrolysis; maximum oligosaccharide production was observed when fructose equivalent (FE) reached 27-48 with a structural profile analysed by high-performance anion-exchange chromatography HPAEC-PAD. After hydrolysis, glucose and fructose may be eliminated through a biological purification step involving the addition of Pichia pastoris cells, which selectively consume these sugars but are unable to metabolise fructooligosaccharides.
设计了一种用于龙舌兰（龙舌兰韦伯蓝变种）果聚糖的可控酸热水解工艺，以生产功能性益生元低聚果糖和甜味剂的混合物。尽管其结构高度分支，但发现其底物浓度遵循一级动力学行为，活化能为95kJ/mol，这与其他线性果聚糖（如菊苣菊粉）的值相似。引入了果糖当量（FE），这是淀粉工业中使用的葡萄糖当量（DE）的类似参数，用于表征果聚糖水解；当果糖当量（FE）达到27-48时，观察到低聚糖产量最高，并通过高效阴离子交换色谱HPAEC-PAD分析其结构特征。水解后，葡萄糖和果糖可通过生物纯化步骤去除，该步骤包括添加巴斯德毕赤酵母细胞，这些细胞选择性消耗这些糖，但无法代谢低聚果糖。
