Sun Xue, Cui Xiao-Bing, Wen Hong-Mei, Shan Chen-Xiao, Wang Xin-Zhi, Kang An, Chai Chuan, Li Wei
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China.
J Pharm Biomed Anal. 2017 Jul 15;141:19-31. doi: 10.1016/j.jpba.2017.03.003. Epub 2017 Mar 7.
In the present study, the chemical compositions of Atractylodes macrocephala Koidz. (AMK) were analyzed systematically and influence of sulfur fumigation on the chemical profiles was evaluated by ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UFLC-QTOF-MS) combined with multivariate statistical analysis. 52 components were detected from non-fumigated AMK (NF-AMK) and 28 components were newly produced after sulfur fumigation, out of which 59 major peaks were identified. The concentrations of 20 compounds significantly decreased and 37 compounds obviously increased. The potential structural transformation mechanism of terpenoids was explored to illustrate the correlation of the components contents before and after sulfur fumigation. Eight sulfur-containing/dehydrated-integrated atractylenolides that evolved from the NF-AMK were screened out as potential characteristic chemical markers to examine the post-harvest handling procedures of commercial AMK with excessive sulfur fumigation and maintain consistent quality.
在本研究中，对白术（Atractylodes macrocephala Koidz.，AMK）的化学成分进行了系统分析，并通过超快速液相色谱-四极杆-飞行时间质谱联用（UFLC-QTOF-MS）结合多元统计分析评估了硫熏对白术化学特征的影响。从未熏硫白术（NF-AMK）中检测到52种成分，硫熏后新产生了28种成分，其中鉴定出59个主要峰。20种化合物的浓度显著降低，37种化合物明显增加。探索了萜类化合物潜在的结构转化机制，以阐明硫熏前后成分含量的相关性。筛选出8种由未熏硫白术衍生而来的含硫/脱水整合白术内酯，作为潜在的特征化学标志物，用于检测商业白术硫熏过量的采收后处理过程并维持质量一致。
