Asadi Mahsa, Fazeli Mohamad Reza, Sabokbar Azar
Dept of Microbiology, Islamic Azad University, Karaj branch, Karaj, Iran.
Dept of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pathol. 2018 Summer;13(3):301-307. Epub 2018 Sep 12.
Acute microbial diarrheal diseases are the major public health problems in the developing countries. People affected by diarrheal diseases have the lowest financial resources and poorest hygienic facilities. Children under five, primarily in Asian and African countries, are mostly the subjects affected by microbial diseases transmitted through water.The current study aimed at investigating the comparative inhibitory effect of Lactocare (commercial probiotic) on clinical samples and standard strains of .
A total of 20 clinical samples and a standard strain ) were provided by Health Reference Laboratory and Biotechnology Institute, respective ly. In order to confirm the samples, biochemical analysis and the polymerase chain reaction (PCR) were performed on intergenic space. Afterward, agar well diffusion method was performed in order to measure the minimum inhibitory concentration to monitor the antimicrobial activity of Lactocare.
Colony count of V. for the standard strain in 30% and mean for clinical samples in 50% concentration of Lactocare treatment revealed that it would propel to death phase. Since the number of colonies decreased to 100, it was considered that higher concentrations of Lactocare would completely inhibit the growth of .
Probiotics are employed to develop new pharmaceutical preparations and functional foods in order to promote the public health.
急性微生物性腹泻疾病是发展中国家的主要公共卫生问题。受腹泻疾病影响的人群财力资源最少且卫生设施最差。主要在亚洲和非洲国家的五岁以下儿童大多是受通过水传播的微生物疾病影响的对象。本研究旨在调查Lactocare（商业益生菌）对临床样本和标准菌株的比较抑制作用。
健康参考实验室和生物技术研究所分别提供了总共20份临床样本和一株标准菌株。为了确认样本，对基因间隔区进行了生化分析和聚合酶链反应（PCR）。之后，采用琼脂孔扩散法测量最低抑菌浓度，以监测Lactocare的抗菌活性。
在30%浓度的Lactocare处理下，标准菌株的副溶血性弧菌菌落计数以及50%浓度处理下临床样本的菌落计数均值显示其将进入死亡期。由于菌落数降至100，因此认为较高浓度的Lactocare会完全抑制副溶血性弧菌的生长。
益生菌被用于开发新的药物制剂和功能性食品以促进公众健康。
