Sharma Supriya, Gupta Suruchi, Dhar Manoj K, Kaul Sanjana
School of Biotechnology, University of Jammu, Jammu, J&K, India.
Mycobiology. 2018 Dec 21;46(4):370-381. doi: 10.1080/12298093.2018.1538068. eCollection 2018.
Bioactive natural compounds, isolated from fungal endophytes, play a promising role in the search for novel drugs. They are an inspiring source for researchers due to their enormous structural diversity and complexity. During the present study fungal endophytes were isolated from a well-known medicinal shrub, DC. and were explored for their antagonistic and antioxidant potential. an important medicinal shrub with remarkable pharmacological properties, is native to Northern Himalayan region. A total of 131 endophytic fungal isolates belonging to eighteen species and nine genera were obtained from three hundred and thirty surface sterilized segments of different tissues of . The isolated fungi were classified on the basis of morphological and molecular analysis. Diversity and species richness was found to be higher in leaf tissues as compared to root and stem. Antibacterial activity demonstrated that the crude ethyl acetate extract of 80% isolates exhibited significant results against one or more bacterial pathogens. Ethyl acetate extract of was found to have potential antibacterial activity. Significant antioxidant activity was also found in crude ethyl acetate extracts of and . Similarly, antagonistic activity of the fungal endophytes revealed that all antagonists possessed inhibition potential against more than one fungal pathogen. This study is an important step towards tapping endophytic fungal diversity for bioactive metabolites which could be a step forward towards development of novel therapeutic agents.
从真菌内生菌中分离出的生物活性天然化合物在寻找新型药物方面发挥着重要作用。由于其巨大的结构多样性和复杂性，它们是研究人员的一个鼓舞人心的来源。在本研究中，从一种著名的药用灌木中分离出真菌内生菌，并对其拮抗和抗氧化潜力进行了探索。这种重要的药用灌木具有显著的药理特性，原产于喜马拉雅北部地区。从该药用灌木不同组织的330个表面消毒切段中，共获得了131株内生真菌分离株，分属于18个种和9个属。根据形态学和分子分析对分离出的真菌进行了分类。结果发现，与根和茎相比，叶组织中的多样性和物种丰富度更高。抗菌活性表明，80%分离株的粗乙酸乙酯提取物对一种或多种细菌病原体表现出显著效果。该药用灌木的乙酸乙酯提取物具有潜在的抗菌活性。在该药用灌木和其他样本的粗乙酸乙酯提取物中也发现了显著的抗氧化活性。同样，真菌内生菌的拮抗活性表明，所有拮抗剂对一种以上的真菌病原体都具有抑制潜力。这项研究是挖掘内生真菌多样性以获取生物活性代谢物的重要一步，这可能是朝着开发新型治疗剂迈出的一步。
