Zhai Ming-Ming, Qi Feng-Ming, Li Jie, Jiang Chun-Xiao, Hou Yue, Shi Yan-Ping, Di Duo-Long, Zhang Ji-Wen, Wu Quan-Xiang
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou 730000, People's Republic of China.
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou 730000, People's Republic of China.
J Agric Food Chem. 2016 Mar 23;64(11):2298-306. doi: 10.1021/acs.jafc.6b00556. Epub 2016 Mar 14.
The fungus Clonostachys rosea is widely distributed all over the world. The destructive force of this fungus, as a biological control agent, is very strong to lots of plant pathogenic fungi. As part of the ongoing search for antibiotics from fungi obtained from soil samples, the secondary metabolites of C. rosea YRS-06 were investigated. Through efficient bioassay-guided isolation, three new bisorbicillinoids possessing open-ended cage structures, tetrahydrotrichodimer ether (1) and dihydrotrichodimer ether A and B (2 and 3), and 12 known compounds were obtained. Their structures were determined via extensive NMR, HR-ESI-MS, and CD spectroscopic analyses and X-ray diffraction data. Compounds 1-3 are rare bisorbicillinoids with a γ-pyrone moiety. The biological properties of 1-15 were evaluated against six different Gram-positive and Gram-negative bacteria. Bisorbicillinoids, 2-5, and TMC-151 C and E, 14 and 15, showed potent antibacterial activity.
粉红粘帚霉广泛分布于世界各地。作为一种生物防治剂，这种真菌对许多植物病原真菌具有很强的破坏力。作为从土壤样品中获得的真菌中筛选抗生素的一部分，对粉红粘帚霉YRS - 06的次生代谢产物进行了研究。通过高效的生物活性导向分离，得到了三种具有开放式笼状结构的新型双螺吡喃类化合物，四氢三枝孢二聚醚（1）和二氢三枝孢二聚醚A和B（2和3），以及12种已知化合物。通过广泛的核磁共振、高分辨电喷雾质谱和圆二色光谱分析以及X射线衍射数据确定了它们的结构。化合物1 - 3是罕见的带有γ-吡喃酮部分的双螺吡喃类化合物。评估了化合物1 - 15对六种不同革兰氏阳性和革兰氏阴性细菌的生物学特性。双螺吡喃类化合物2 - 5以及TMC - 151 C和E（14和15）表现出强大的抗菌活性。
