Yousfi Khadidja, Touati Abdelaziz, Lefebvre Brigitte, Garneau Philippe, Brahmi Soumia, Gharout-Sait Alima, Harel Josée, Bekal Sadjia
Laboratoire d'Écologie Microbienne, FSNV, Université de Béjaia, 06000, Béjaia, Algeria.
Laboratoire de santé publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, QC, H9X 3R5, Canada.
Braz J Microbiol. 2019 Jan;50(1):175-183. doi: 10.1007/s42770-018-0010-9. Epub 2018 Dec 19.
The antibiotic susceptibility profile and antimicrobial resistance determinants were characterized on Gram-negative bacilli (GNB) isolated from Algerian hospital effluents. Among the 94 isolates, Enterobacteriaceae was the predominant family, with Escherichia coli and Klebsiella pneumoniae being the most isolated species. In non-Enterobacteriaceae, Acinetobacter and Aeromonas were the predominant species followed by Pseudomonas, Comamonas, Pasteurella, and Shewanella spp. The majority of the isolates were multidrug-resistant (MDR) and carried different antimicrobial resistance genes including bla, bla, bla, bla-like, bla, bla, qnrB, qnrS, tet(A), tet(B), tet(C), dfrA1, aac(3)-IIc (aacC2), aac(6')-1b, sul1, and sul2. The qacEΔ1-sul1 and intI2 signatures of class 1 and class 2 integrons, respectively, were also detected. Microarray hybridization on MDR E. coli revealed additional resistance genes (aadA1 and aph3strA, tet30, mphA, dfrA12, bla, bla, and cmlA1) and classified the tested strains as commensals, thus highlighting the potential role of humans in antibiotic resistance dissemination. This study is the first report of bla-like in Klebsiella oxytoca in Algeria and bla in A. baumannii in Algerian hospital effluents. The presence of these bacteria and resistance genes in hospital effluents represents a serious public health concern since they can be disseminated in the environment and can colonize other hosts.
对从阿尔及利亚医院污水中分离出的革兰氏阴性杆菌（GNB）的抗生素敏感性谱和抗菌耐药性决定因素进行了表征。在94株分离菌中，肠杆菌科是主要菌科，大肠杆菌和肺炎克雷伯菌是分离最多的菌种。在非肠杆菌科中，不动杆菌属和气单胞菌属是主要菌种，其次是假单胞菌属、丛毛单胞菌属、巴斯德菌属和希瓦氏菌属。大多数分离菌具有多重耐药性（MDR），并携带不同的抗菌耐药基因，包括bla、bla、bla、bla样、bla、bla、qnrB、qnrS、tet(A)、tet(B)、tet(C)、dfrA1、aac(3)-IIc（aacC2）、aac(6')-1b、sul1和sul2。还分别检测到了1类和2类整合子的qacEΔ1-sul1和intI2特征。对多重耐药大肠杆菌进行的微阵列杂交揭示了其他耐药基因（aadA1和aph3strA、tet30、mphA、dfrA12、bla、bla和cmlA1），并将测试菌株分类为共生菌，从而突出了人类在抗生素耐药性传播中的潜在作用。本研究首次报道了阿尔及利亚医院污水中产酸克雷伯菌中的bla样基因以及鲍曼不动杆菌中的bla基因。医院污水中这些细菌和耐药基因的存在代表了严重的公共卫生问题，因为它们可以在环境中传播并能定殖于其他宿主。
