Allahmoradi Esmaeil, Taghiloo Saeid, Omrani-Nava Versa, Shobeir Saeideh Sadat, Tehrani Mohsen, Ebrahimzadeh Mohammad Ali, Asgarian-Omran Hossein
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Med J Islam Repub Iran. 2018 Sep 3;32:80. doi: 10.14196/mjiri.32.80. eCollection 2018.
, known as Purslane, is an annual growing herb with wide distribution around the world and traditionally used to manage several diseases. Different therapeutic properties as an anti-fever agent as well as anti-inflammatory and analgesic effects have been attributed to . The aim of this study was to investigate the effects of aerial extract on production of pro- and anti-inflammatory cytokines by human peripheral blood mononuclear cells (PBMCs). Aerial parts of (stems and leaves) were collected and extracted by percolation using methanol. The optimal and non-cytotoxic dose of hydro-alcoholic extract for cell culture analysis was determined by MTT assay. To assess the antiinflammatory effects of , PBMCs obtained from 12 normal volunteers were cultured in RPMI complete medium and cotreated with E. coli lipopolysaccharide (LPS) and hydro-alcoholic extract. Following 18-hour incubation, culture supernatants were harvested for measurement of secreted TNF-α, IL-6 and IL-10 by ELISA. Statistical analyses were performed using the SPSS v.20, and data analyzed by Kolmogorov-Smirnov, Mann-Whitney U, Kruskal-Wallis and post Hoc tests. P-values<0.05 were considered significant. The optimal non-cytotoxic concentration of aerial extract was defined as 100 μg/ml based on MTT viability assay. hydro-alcoholic extract significantly decreased the concentration of both pro-inflammatory cytokines TNF-α and IL-6 in LPS-stimulated PBMCs (p<0.001 and p<0.001, respectively). However, the concentration of IL-10 as an anti-inflammatory cytokine, did not show any statistically significant change (p=0.390). Our findings highlighted the potential anti-inflammatory properties of in herbal medicine. Future analysis on different constituents of total extract may confirm its therapeutic effects as a promising anti-inflammatory compound.
马齿苋是一种一年生草本植物，在全球广泛分布，传统上用于治疗多种疾病。马齿苋具有不同的治疗特性，如作为退烧药以及抗炎和镇痛作用。本研究的目的是调查马齿苋地上部分提取物对人外周血单核细胞（PBMCs）促炎和抗炎细胞因子产生的影响。马齿苋的地上部分（茎和叶）被收集并通过甲醇渗漉法提取。通过MTT法确定用于细胞培养分析的水醇提取物的最佳非细胞毒性剂量。为了评估马齿苋的抗炎作用，从12名正常志愿者获得的PBMCs在RPMI完全培养基中培养，并与大肠杆菌脂多糖（LPS）和马齿苋水醇提取物共同处理。孵育18小时后，收集培养上清液，通过ELISA测量分泌的TNF-α、IL-6和IL-10。使用SPSS v.20进行统计分析，并通过Kolmogorov-Smirnov、Mann-Whitney U、Kruskal-Wallis和事后检验分析数据。P值<0.05被认为具有统计学意义。基于MTT活力测定，马齿苋地上部分提取物的最佳非细胞毒性浓度定义为100μg/ml。马齿苋水醇提取物显著降低了LPS刺激的PBMCs中促炎细胞因子TNF-α和IL-6的浓度（分别为p<0.001和p<0.001）。然而，作为抗炎细胞因子的IL-10的浓度没有显示出任何统计学上的显著变化（p=0.390）。我们的研究结果突出了马齿苋在草药医学中的潜在抗炎特性。对总提取物不同成分的进一步分析可能会证实其作为一种有前景的抗炎化合物的治疗效果。
