基于主成分分析的水性多草药组合的开发及其在 HepG2 细胞中的功能意义。
Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells.
机构信息
Department of Botanical & Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Cancer Biology Division, IIT Research Institute and Department of Biological Sciences, Illinois Institute of Technology, Chicago, IL, 60616, USA.
出版信息
BMC Complement Altern Med. 2019 Jan 15;19(1):18. doi: 10.1186/s12906-019-2432-9.
BACKGROUND
The present study was carried out to prepare multi-herbal combination via comparing antioxidant activity and polyphenolic composition of five medicinal plant extracts of Punica granatum L., Putranjiva roxburghii Wall., Swertia chirata Buch.-Ham., Tinospora cordifolia (Willd.) Miers and Trigonella corniculata L.
METHODS
The herbs were individually evaluated using in vitro antioxidant assays and analyzed by HPLC-PDA. The resultant data was examined using principal component analysis (PCA). Further, herbal combination was prepared on the basis of PCA.
RESULTS
The PCA divided the plants into three groups. The leading or primary group contained P. granatum and P. roxburghii with the highest antioxidant activity strongly correlated with high amount of kaempferol. S. chirata was acknowledged as nourisher herb in one and T. cordifolia and T. corniculata were identified as stimulator herbs in other group. The herbal combination exhibited high antioxidant activity as compared to the individual plants. The combination revealed good antiproliferative efficacy against hepatocellular carcinoma (HepG2) cells with IC of 75.864 μg/ml.
CONCLUSIONS
The activity observed in vitro with HepG2 cells suggests that the herbal combination can provide therapeutic activity in vivo in future. The study may provide information regarding precise preparation of multi-herbal formulations using PCA as a tool in pharmaceutical industries.
背景
本研究通过比较石榴皮、余甘子、当药、三叶鬼针草和胡芦巴五种药用植物提取物的抗氧化活性和多酚组成，制备了多草药组合。
方法
采用体外抗氧化试验对各草药进行评价，并采用高效液相色谱-光电二极管阵列（HPLC-PDA）进行分析。使用主成分分析（PCA）对所得数据进行检验。在此基础上，基于 PCA 制备了草药组合。
结果
PCA 将植物分为三组。主导或主要组包含具有最高抗氧化活性的石榴皮和余甘子，其与高水平的山柰酚强烈相关。当药被认为是一种滋补草药，三叶鬼针草和胡芦巴则分别被鉴定为另一种和第三种刺激草药。与单一植物相比，草药组合表现出更高的抗氧化活性。该组合对肝癌（HepG2）细胞表现出良好的抗增殖作用，IC 为 75.864μg/ml。
结论
体外与 HepG2 细胞的活性观察表明，该草药组合在未来可能具有体内治疗活性。该研究可能为利用 PCA 作为制药行业的工具精确制备多草药配方提供信息。
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