Kalyana Sundaram Induja, Sarangi Deepika Deeptirekha, Sundararajan Vignesh, George Shinomol, Sheik Mohideen Sahabudeen
Department of Biotechnology, Dayanandasagar College of Engineering, Kumaraswamy Layout, Bangalore, 560078, Karnataka, India.
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India.
BMC Complement Altern Med. 2018 Jan 29;18(1):33. doi: 10.1186/s12906-018-2097-9.
Skin forms an important part of human innate immune system. Wrinkles, thinning and roughening of skin are some of the symptoms that affect the skin as it ages. Reactive oxygen species induced oxidative stress plays a major role in skin aging by modulating the elastase enzyme level in the skin. Extrinsic factors that affect skin aging such as UV radiation can also cause malignant melanoma. Here we selected four medicinal plant materials, namely, leaves of Nyctanthes arbor-tristis, unripe and ripe Aegle marmelos fruit pulp and the terminal meristem of Musa paradisiaca flower and investigated their anti-aging properties and cytotoxicity in vitro individually as well as in a poly herbal formulation containing the four plant extracts in different ratios.
The phytochemical contents of the plant extracts were investigated for radical scavenging activity and total reducing power. Based upon its anti-oxidant properties, a poly herbal formulation containing leaves of Nyctanthes arbor-tristis, unripe and ripe fruit pulp of Aegle marmelos, and the terminal meristem of Musa paradisiaca flower in the ratio 6:2:1:1 (Poly Herbal Formulation 1) and 1:1:1:1 (Poly Herbal Formulation 2), respectively were formulated.
It has been observed that the Poly Herbal Formulation 1 was more potent than Poly Herbal Formulation 2 due to better anti-oxidant and anti-elastase activities in NIH3T3 fibroblast cells. In addition Poly Herbal formulation 1 also had better anti-cancer activity in human malignant melanoma cells.
Based on these results these beneficial plant extracts were identified for its potential application as an anti-aging agent in skin creams as well as an anti-proliferation compound against cancer cells.
皮肤是人类先天免疫系统的重要组成部分。皱纹、皮肤变薄和粗糙是皮肤老化时出现的一些症状。活性氧诱导的氧化应激通过调节皮肤中的弹性蛋白酶水平在皮肤老化过程中起主要作用。影响皮肤老化的外在因素，如紫外线辐射，也可导致恶性黑色素瘤。在此，我们选择了四种药用植物材料，即夜花树的叶子、印度枳椇未成熟和成熟的果肉以及香蕉花的顶端分生组织，并分别研究了它们的体外抗衰老特性和细胞毒性，以及一种含有这四种植物提取物不同比例的多草药配方。
研究了植物提取物的植物化学成分的自由基清除活性和总还原能力。基于其抗氧化特性，分别配制了一种多草药配方，其中含有夜花树的叶子、印度枳椇未成熟和成熟的果肉以及香蕉花的顶端分生组织，比例分别为6:2:1:1（多草药配方1）和1:1:1:1（多草药配方2）。
已观察到，由于在NIH3T3成纤维细胞中具有更好的抗氧化和抗弹性蛋白酶活性，多草药配方1比多草药配方2更有效。此外，多草药配方1在人恶性黑色素瘤细胞中也具有更好的抗癌活性。
基于这些结果，确定了这些有益的植物提取物作为抗衰老剂在护肤霜中的潜在应用以及作为抗癌细胞的抗增殖化合物的潜在应用。
