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伊朗开心果的表型和基因型变异。

Phenotypic and genotypic variation in Iranian Pistachios.

作者信息

Aliakbarkhani Somayeh Tayefeh, Akbari Mohammad, Hassankhah Amin, Talaie Alireza, Moghadam Mohammadreza Fattahi

机构信息

Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Department of Horticulture, College of Abouraihan, University of Tehran, Tehran, Iran.

出版信息

J Genet Eng Biotechnol. 2015 Dec;13(2):235-241. doi: 10.1016/j.jgeb.2015.05.003. Epub 2015 Jun 13.

Abstract

As Iran is one of the richest pistachio germplasms a few studies have been conducted on different sexes of pistachio trees, in areas where this crop emerged. To this end, 40 male and female Iranian pistachio genotypes from Feizabad region, Khorasan, Iran; were evaluated using morphological characters and randomly amplified polymorphic DNA (RAPD) markers. For morphological assessments, 54 variables were considered to investigate similarities between and among the studied genotypes. Morphological data indicated relative superiority in some female genotypes (such as Sefid 1, Sefid Sabuni 2, Garmesiah, and Ghermezdorosht Z) regarding characters such as halfcrackedness, the percentages of protein and fat content. 115 polymorphic bands were recorded with 92.83% average polymorphism among all primers. The total resolving power (Rp) of the primers was 74.54. The range of genetic similarity varied from about 0.31 to about 0.70. Genotypes were segregated into eight groups at the similarity limit of 0.41. Results of present investigation could be helpful for strategic decisions for maintaining Iranian pistachio genotypes.

摘要

由于伊朗是拥有最丰富开心果种质资源的地区之一,因此在开心果作物起源的地区,针对开心果树的不同性别开展了一些研究。为此,对来自伊朗霍拉桑省费扎巴德地区的40个伊朗开心果雌雄基因型进行了评估,评估采用形态学特征和随机扩增多态性DNA(RAPD)标记。对于形态学评估,考虑了54个变量以研究各研究基因型之间的相似性。形态学数据表明,一些雌性基因型(如Sefid 1、Sefid Sabuni 2、Garmesiah和Ghermezdorosht Z)在半开裂度、蛋白质和脂肪含量百分比等特征方面具有相对优势。在所有引物中,共记录到115条多态性条带,平均多态性为92.83%。引物的总分辨能力(Rp)为74.54。遗传相似性范围约为0.31至0.70。在相似性阈值为0.41时,基因型被分为八组。本研究结果有助于制定维护伊朗开心果基因型的战略决策。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5a9/6299885/13e7b552e6ed/gr1.jpg

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