Moustafa Mahmoud F, Hesham Abd El-Latif, Quraishi Manal S, Alrumman Sulaiman A
Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
Botany Department, Faculty of Science, South Valley University, Qena, Egypt.
J Genet Eng Biotechnol. 2016 Dec;14(2):349-362. doi: 10.1016/j.jgeb.2016.09.001. Epub 2016 Oct 10.
Altitudinal gradient-defined specific environmental conditions could lead to genetics and chemical variations among individuals of the same species. By using RAPD, ISSR, GC-MS and HPLC analysis, the genetic and chemical diversity of plants at various altitudinal gradient namely; Abha (2227.86 m), Dala Valley (1424 m), Rakhma Valley (1000 m), Raheb Valley (505 m) and Al-Marbh (147 m) were estimated. RAPD markers revealed that the highest similarity value (40.22%) was between Raheb Valley and Al-Marbh while the lowest similarity (10.08%) was between Abha and Raheb Valley. Based on ISSR markers the highest similarity value (61.54%) was also between Raheb Valley and Al-Marbh, while the lowest similarity (26.84%) was between Abha and Rakhma Valley. GC-MS results showed the presence of various phytochemical constituents in each population. The dendrogram based on chemical compounds separated the grown at the highest elevations (Abha) from the populations in lower elevations. HPLC analysis showed that the leaves of plant contain considerable amount of vitamins including B, B, B and folic acid. In conclusion there is a close relation between altitudinal gradients, genetic diversity and chemical constituents of the leaves of plants.
海拔梯度定义的特定环境条件可能导致同一物种个体之间的遗传和化学差异。通过使用随机扩增多态性DNA（RAPD）、简单序列重复区间（ISSR）、气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）分析，对不同海拔梯度下的植物进行了遗传和化学多样性评估，这些海拔梯度分别为：阿卜哈（2227.86米）、达拉谷（1424米）、拉克马谷（1000米）、拉赫卜谷（505米）和马尔卜（147米）。RAPD标记显示，拉赫卜谷和马尔卜之间的相似性值最高（40.22%），而阿卜哈和拉赫卜谷之间的相似性最低（10.08%）。基于ISSR标记，拉赫卜谷和马尔卜之间的相似性值也最高（61.54%），而阿卜哈和拉克马谷之间的相似性最低（26.84%）。GC-MS结果表明每个种群中存在各种植物化学成分。基于化合物的聚类图将生长在最高海拔（阿卜哈）的植物与低海拔种群分开。HPLC分析表明，该植物的叶子含有大量维生素，包括维生素B、B、B和叶酸。总之，海拔梯度、遗传多样性与该植物叶子的化学成分之间存在密切关系。
