Department of Industrial Biotechnology, Bharath University, Chennai 600 073, Tamil Nadu, India.
Department of Electronics and Material Science, Research Institute of Electronics, Shizuoka University, Hamamatsu 432-8011, Japan.
J Food Drug Anal. 2018 Apr;26(2):917-925. doi: 10.1016/j.jfda.2017.07.014. Epub 2017 Aug 31.
Green synthetic route of silver nanoparticles (AgNPs) has already been proved to be an advantageous over other physico-chemical approaches due to its simplicity, cost effectiveness, ecofriendly and nontoxicity. In this finding, aqueous Wedelia chinensis leaf extract (WLE) mediated synthesis of AgNPs was approached. Surface plasmon resonance (SPR) band at 408 nm preliminary indicated the formation of AgNPs, while TEM and XRD characterization confirmed the formation of spherically shaped and crystalline AgNPs with an average size of 31.68 nm, respectively. The plausible biomolecules in the aqueous leaf extract responsible for the reduction and stabilization of AgNPs were identified by FTIR analysis and found to be polyphenolic groups in flavonoid. Further, synthesized AgNPs was explored for different biological applications. Biosynthesized AgNPs showed significant free radical scavenging activity as compared to Wedelia leaf extract and antibacterial activity against clinically isolated test pathogens where Gram-negative bacteria were found more susceptible to AgNPs than Gram-positive one. In addition, in vitro cytotoxic response was also evaluated on hepatocellular carcinoma Hep G2 cell lines and showed a dose-dependent cytotoxic response with an IC value of 25 μg/mL.
绿色的银纳米粒子（AgNPs）的合成路线已经被证明是优于其他物理化学方法，因为它的简单、成本效益高、环保和无毒。在这一发现中，采用了水蜈蚣叶提取物（WLE）介导的 AgNPs 合成方法。408nm 的表面等离子体共振（SPR）带初步表明 AgNPs 的形成，而 TEM 和 XRD 特性分析则分别证实了球形和结晶 AgNPs 的形成，平均粒径为 31.68nm。通过傅里叶变换红外（FTIR）分析鉴定出负责还原和稳定 AgNPs 的水相叶提取物中的生物分子，发现它们是类黄酮中的多酚类。此外，还探索了合成的 AgNPs 在不同的生物应用。与水蜈蚣叶提取物相比，生物合成的 AgNPs 表现出显著的自由基清除活性，并且对临床分离的测试病原体具有抗菌活性，其中革兰氏阴性菌比革兰氏阳性菌对 AgNPs 更敏感。此外，还在肝细胞癌 Hep G2 细胞系上评估了体外细胞毒性反应，并表现出剂量依赖性的细胞毒性反应，IC 值为 25μg/mL。
