Ahmadipour A, Sharififar F, Nakhaipour F, Samanian M, Karami-Mohajeri S
Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
J Med Life. 2015;8(Spec Iss 4):275-281.
This research aimed to evaluate the protective effects of methanolic extract of Zataria Multiflora Boiss (Z. Multiflora) against hepatic damage induced by cisplatin in male Wistar rats. Hepatotoxicity was induced in Wistar male rats by a single intraperitoneal administration of cisplatin, 7 g/ kg body weight. A methanolic extract of Z. Multiflora was administered orally at doses of 50 mg/ kg, 100 mg/ kg, 200 mg/ kg and 400 mg/ kg body weight daily for seven days after being cisplatin-induced. The study included the histopathological examination of the liver sections. The activity of aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) were evaluated as markers of liver damage. The superoxide dismutase (SOD), the activity of Catalase (CAT), and glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) and nitric oxide (NO) content in serum were measured as an oxidative stress factor. The results showed that rat treated with cisplatin resulted in a significant increase in serum activity, AST, ALT and ALP in treated mice. Management with Z. Multiflora reduced the business of these enzymes to nearly normal levels. In parallel with these changes, this extract reduced cisplatin-induced oxidative stress by inhibiting lipid peroxidation and protein carbonylation, and restoring the antioxidant enzyme (SOD, CAT, and GSH-Px) and elevation of the glutathione level. Biochemical and histological observations showed the hepatoprotective effect was found in a dose-dependent manner in Z. Multiflora methanolic extract. This protective effect can be attributed to the antioxidant compounds.
本研究旨在评估百里香提取物对顺铂诱导的雄性Wistar大鼠肝损伤的保护作用。通过腹腔注射7mg/kg体重的顺铂诱导Wistar雄性大鼠产生肝毒性。在顺铂诱导后，每天以50mg/kg、100mg/kg、200mg/kg和400mg/kg体重的剂量口服百里香甲醇提取物，持续7天。该研究包括对肝脏切片进行组织病理学检查。评估天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）的活性作为肝损伤的标志物。测量血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的活性以及丙二醛（MDA）和一氧化氮（NO）的含量作为氧化应激因子。结果表明，顺铂处理的大鼠血清中AST、ALT和ALP活性显著增加。百里香处理使这些酶的活性降低至接近正常水平。与这些变化同时，该提取物通过抑制脂质过氧化和蛋白质羰基化，恢复抗氧化酶（SOD、CAT和GSH-Px）以及提高谷胱甘肽水平，减轻了顺铂诱导的氧化应激。生化和组织学观察表明，百里香甲醇提取物具有剂量依赖性的肝保护作用。这种保护作用可归因于抗氧化化合物。
